ArticleBMC cell biology2008
Spatial separation and bidirectional trafficking of proteins using a multi-functional reporter.
Article in BMC cell biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Nanoscale architecture and dynamics of CaJournal of molecular and cellular cardiology plus · 2025Article
- Optimizing multifunctional fluorescent ligands for intracellular labeling.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- A genetic driver of epileptic encephalopathy impairs gating of synaptic glycolysis.bioRxiv : the preprint server for biology · 2025Article
- Absolute protein quantification using fluorescence measurements with FPCountR.Nature communications · 2022Article
- A self-labeling protein based on the small ultra-red fluorescent protein, smURFP.RSC chemical biology · 2021Article
- Acetylation-dependent regulation of PD-L1 nuclear translocation dictates the efficacy of anti-PD-1 immunotherapy.Nature cell biology · 2020Article
- HaloTag technology: a versatile platform for biomedical applications.Bioconjugate chemistry · 2015Review
- Genetic targeting of a small fluorescent zinc indicator to cell surface for monitoring zinc secretion.ACS chemical biology · 2015Article
- Article
- HaloTag as a reporter gene: positron emission tomography imaging with (64)Cu-labeled second generation HaloTag ligands.American journal of translational research · 2013Article
- Preferential release of newly synthesized insulin assessed by a multi-label reporter system using pancreatic β-cell line MIN6.PloS one · 2012Article
- Development of a dehalogenase-based protein fusion tag capable of rapid, selective and covalent attachment to customizable ligands.Current chemical genomics · 2012Article
- HaloTag, a Platform Technology for Protein Analysis.Current chemical genomics · 2012Article
- Galectin-1 and Galectin-3 Mediate Protocadherin-24-Dependent Membrane Localization of β-catenin in Colon Cancer Cell Line HCT116.Current chemical genomics · 2012Article
- HaloTag: a novel reporter gene for positron emission tomography.American journal of translational research · 2011Article
- A functional analysis of the CREB signaling pathway using HaloCHIP-chip and high throughput reporter assays.BMC genomics · 2009Article
- Illuminating the life of GPCRs.Cell communication and signaling : CCS · 2009Article
- In vivo stable tumor-specific painting in various colors using dehalogenase-based protein-tag fluorescent ligands.Bioconjugate chemistry · 2009Article
- In vivo labeling method using a genetic construct for nanoscale resolution microscopy.Biophysical journal · 2009Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe ability to specifically label proteins within living cells can provide information about their dynamics and function. To study a membrane protein, we fused a multi-functional reporter protein, HaloTag, to the extracellular domain of a truncated integrin.
resultsUsing the HaloTag technology, we could study the localization, trafficking and processing of an integrin-HaloTag fusion, which we showed had cellular dynamics consistent with native integrins. By labeling live cells with different fluorescent impermeable and permeable ligands, we showed spatial separation of plasma membrane and internal pools of the integrin-HaloTag fusion, and followed these protein pools over time to study bi-directional trafficking. In addition to combining the HaloTag reporter protein with different fluorophores, we also employed an affinity tag to achieve cell capture.
conclusionThe HaloTag technology was used successfully to study expression, trafficking, spatial separation and real-time translocation of an integrin-HaloTag fusion, thereby demonstrating that this technology can be a powerful tool to investigate membrane protein biology in live cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.