ArticleBMC physiology2008
Bridging the phenotypic gap: real-time assessment of mitochondrial function and metabolism of the nematode Caenorhabditis elegans.
Article in BMC physiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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47 citing papers in PubMed, 81 citations in OpenAlex.
- Article
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- An Intricate Network Involving the Argonaute ALG-1 Modulates Organismal Resistance to Oxidative Stress.Nature communications · 2024Article
- Bioluminescence as a functional tool for visualizing and controlling neuronal activityNeurophotonics · 2024Article
- The mitochondrial calcium uniporter (MCU) activates mitochondrial respiration and enhances mobility by regulating mitochondrial redox state.Redox biology · 2023Article
- Examining Sporadic Cancer Mutations Uncovers a Set of Genes Involved in Mitochondrial Maintenance.Genes · 2023Article
- In vivo quantitative high-throughput screening for drug discovery and comparative toxicology.Disease models & mechanisms · 2023Article
- Deviations from temporal scaling support a stage-specific regulation for C. elegans postembryonic development.BMC biology · 2022Article
- NanoBRET in C. elegans illuminates functional receptor interactions in real time.BMC molecular and cell biology · 2022Article
- Guaiazulene derivative 1,2,3,4-tetrahydroazuleno[1,2-b] tropone reduces the production of ATP by inhibiting electron transfer complex II.FEBS open bio · 2021Article
- Mitochondria-affecting small molecules ameliorate proteostasis defects associated with neurodegenerative diseases.Scientific reports · 2021Article
- Insulin/IGF-1 signaling and heat stress differentially regulate HSF1 activities in germline development.Cell reports · 2021Article
- Multiple metabolic changes mediate the response of Caenorhabditis elegans to the complex I inhibitor rotenone.Toxicology · 2021Article
- Article
- Developmental function and state transitions of a gene expression oscillator in Caenorhabditis elegans.Molecular systems biology · 2020Article
- A salt-induced kinase is required for the metabolic regulation of sleep.PLoS biology · 2020Article
- Nanoluciferase-Based Method for Detecting Gene Expression inGenetics · 2019Article
- Cell-autonomous and non-autonomous roles ofAging · 2019Article
- Purine Homeostasis Is Necessary for Developmental Timing, Germline Maintenance and Muscle Integrity inGenetics · 2019Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe ATP levels of an organism are an important physiological parameter that is affected by genetic make up, ageing, stress and disease.
resultsWe have generated luminescent C. elegans through ubiquitous, constitutive expression of firefly luciferase, widely used for in vitro ATP determination. We hypothesise that whole animal luminescence reflects its intracellular ATP levels in vivo. To test this, we characterised the bioluminescence response of C. elegans during sublethal exposure to, and recovery from azide, a treatment that inhibits mitochondrial respiration reversibly, and causes ATP depletion. Consistent with our expectations, in vivo luminescence decreased with increasing sublethal azide levels, and recovered fully when worms were removed from azide. Firefly luciferase expression levels, stability and activity did not influence the final luminescence. Bioluminescence also reflected the lowered activity of the electron transport chain achieved with RNA interference (RNAi) of genes encoding respiratory chain components.
conclusionResults indicated that C. elegans luminescence reports on ATP levels in real-time. For the first time, we are able to directly assess the metabolism of a whole, living, multicellular organism by determination of the relative ATP levels. This will enable genetic analysis based on a readily quantifiable metabolic phenotype and will provide novel insights into mechanisms of fitness and disease that are likely to be of relevance for other organisms, as well as the worm.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.