ArticleClinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology2008
IL-13 induced increases in nitrite levels are primarily driven by increases in inducible nitric oxide synthase as compared with effects on arginases in human primary bronchial epithelial cells.
Article in Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. It is linked to trial NCT05870215 (Phenotyping Responses to Systemic Corticosteroids in the Management of Asthma Attacks), which is not on this map. Cited by 93 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Phenotyping Responses to Systemic Corticosteroids in the Management of Asthma Attacks (PRISMA): Clinical and Translational Correlation to Point-Of-Care Biomarkers
Who cites it
93 citing papers in PubMed, 2 syntheses or guidelines pooled it, 205 citations in OpenAlex.
- Dupilumab efficacy and safety in patients with moderate to severe asthma: A systematic review and meta-analysis.Frontiers in pharmacology · 2022Pooled it
- Efficacy and safety of dupilumab for the treatment of uncontrolled asthma: a meta-analysis of randomized clinical trials.Respiratory research · 2019Pooled it
- Effect of Buteyko breathing technique on clinical and functional parameters in adult patients with asthma: a randomized, controlled study.European journal of medical research · 2024Trial
- Application of structured statistical analyses to identify a biomarker predictive of enhanced tralokinumab efficacy in phase III clinical trials for severe, uncontrolled asthma.BMC pulmonary medicine · 2019Trial
- Randomized study of the safety and pharmacodynamics of inhaled interleukin-13 monoclonal antibody fragment VR942.EBioMedicine · 2018Trial
- A phase 1, randomized, placebo-controlled, dose-escalation study of an anti-IL-13 monoclonal antibody in healthy subjects and mild asthmatics.British journal of clinical pharmacology · 2013Trial
- Short-term longitudinal variation in exhaled nitric oxide fraction predicts future moderate-to-severe exacerbations in COPD patients on triple therapy: a prospective cohort study.ERJ open research · 2026Article
- Fractional exhaled nitric oxide and blood eosinophils in relation to lung function, allergy and nasal polyps in asthma.The journal of allergy and clinical immunology. Global · 2026Article
- Relationships between basement membrane zone thickness and airway physiology, epithelial gene expression and innate immune cell infiltration in asthma.ERJ open research · 2026Article
- HAntioxidants (Basel, Switzerland) · 2026Article
- Long-term effectiveness and safety of benralizumab in EGPA: a 3-year single-center experience.Annals of medicine · 2025Article
- Inflammation in Asthma: Mechanistic Insights and the Role of Biologics in Therapeutic Frontiers.Biomedicines · 2025Review
- Clinical and Biological Features of a Thickened Basement Membrane Zone in Asthma.American journal of respiratory and critical care medicine · 2025Article
- Biologics alter the relationship between exhaled nitric oxide fraction and sputum eosinophils in severe T2-high asthma patients.ERJ open research · 2025Article
- Review
- The epithelial era of asthma research: knowledge gaps and future direction for patient care.European respiratory review : an official journal of the European Respiratory Society · 2024Review
- Asthma Pathogenesis: Phenotypes, Therapies, and Gaps: Summary of the Aspen Lung Conference 2023.American journal of respiratory cell and molecular biology · 2024Review
- Association of IL-33 in modeling type-2 airway inflammation and pulmonary emphysema in mice.Immunity, inflammation and disease · 2024Article
- SURFACTANT PROTEIN A: AN INNATE IMMUNE MODULATOR AND THERAPEUTIC IN ASTHMA.Transactions of the American Clinical and Climatological Association · 2024Review
- Immunologic aspects of asthma: from molecular mechanisms to disease pathophysiology and clinical translation.Frontiers in immunology · 2024Review
33 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issuedMustovitch, A T [corrected to Mustovich, A T]
Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundExhaled nitric oxide is increased in asthma, but the mechanisms controlling its production, including the effects of T-helper type 2 (Th2) cytokines, are poorly understood. In mouse and submerged human epithelial cells, Th2 cytokines inhibit expression of inducible nitric oxide synthase (iNOS). Arginases have been proposed to contribute to asthma pathogenesis by limiting the arginine substrate available to NOS enzymes, but expression of any of these enzymes has not been extensively studied in primary human cells.
objectivesWe hypothesized that primary human airway epithelial cells in air-liquid interface (ALI) culture would increase iNOS expression and activity in response to IL-13, while decreasing arginase expression.
methodsiNOS and arginase mRNA (real-time PCR) and protein expression (Western blot and immunofluorescence) as well as iNOS activity (nitrite levels) were measured in ALI epithelial cells cultured from bronchial brushings of normal and asthmatic subjects following IL-13 stimulation.
resultsIL-13 up-regulated iNOS mRNA primarily at a transcriptional level in epithelial cells. iNOS protein and activity also increased, arginase1 protein expression decreased while arginase 2 expression did not change. The changes in iNOS protein correlated strongly with changes in nitrites, and inclusion of arginase (1 or 2) did not substantially change the relationship. Interestingly, iNOS mRNA and protein were not correlated.
conclusionsThese results contrast with many previous results to confirm that Th2 stimuli enhance iNOS expression and activity. While arginase 1 protein decreases in response to IL-13, neither arginase appears to substantially impact nitrite levels in this system.
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Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.