ArticleProceedings of the National Academy of Sciences of the United States of America2008
DNA surface hybridization regimes.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
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Who cites it
63 citing papers in PubMed.
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- Constant Temperature Electrochemical Biosensor for SNP Detection in Human Genomic DNA Based on DNA Melting Analysis.ACS sensors · 2025Article
- Single-Base Detection of DNA with Simplified Steps on InGaN Quantum Wells.The journal of physical chemistry. B · 2025Article
- Design and Validation of Specific Oligonucleotide Probes on Planar Magnetic Biosensors.Analytical chemistry · 2024Article
- Analyzing Binding Specificity in a Microparticle-Based DNA Displacement Assay Using Multiharmonic QCM-D.Langmuir : the ACS journal of surfaces and colloids · 2024Article
- Applications of Surface Plasmon Resonance for Advanced Studies Involving Nucleic Acids.RNA nanomed · 2024Article
- Molecular Dynamics Simulations of the miR-155 Duplex: Impact of Ionic Strength on Structure and NaMolecules (Basel, Switzerland) · 2024Article
- A Careful Insight into DDI-Type Receptor Layers on the Way to Improvement of Click-Biology-Based Immunosensors.Biosensors · 2024Article
- Detection of miR-155 Using Peptide Nucleic Acid at Physiological-like Conditions by Surface Plasmon Resonance and Bio-Field Effect Transistor.Biosensors · 2024Article
- Hybridization Kinetics of miR-155 on Gold Surfaces as Investigated by Surface Plasmon Resonance and Atomic Force Spectroscopy.ACS omega · 2023Article
- FRET Imaging of Nonuniformly Distributed DNA SAMs on Gold Reveals the Role Played by the Donor/Acceptor Ratio and the Local Environment in Measuring the Rate of Hybridization.Chemical & biomedical imaging · 2023Article
- GeomBD3: Brownian Dynamics Simulation Software for Biological and Engineered Systems.Journal of chemical information and modeling · 2022Article
- A Multiplexable Plasmonic Hairpin-DNA Sensor Based On Target-specific Tether Dynamics.ACS sensors · 2021Article
- Electrochemical Beacon Method to Quantify 10 Attomolar Nucleic Acids with a Semilog Dynamic Range of 7 Orders of Magnitude.Analytical chemistry · 2021Article
- Article
- Organic and inorganic mixed phase modification of a silver surface for functionalization with biomolecules and stabilization of electromotive force.RSC advances · 2021Article
- DNA Transformations for Diagnosis and Therapy.Advanced functional materials · 2021Review
- Modeling Effects of Surface Properties and Probe Density for Nanoscale Biosensor Design: A Case Study of DNA Hybridization near Surfaces.The journal of physical chemistry. B · 2021Article
- Label-Free Homogeneous microRNA Detection in Cell Culture Medium Based on Graphene Oxide and Specific Fluorescence Quenching.Nanomaterials (Basel, Switzerland) · 2021Article
3 more citing papers are in PubMed but not listed here.
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Authors and funding
2 authors.
Funding
Abstract
Surface hybridization reactions, in which sequence-specific recognition occurs between immobilized and solution nucleic acids, are routinely carried out to quantify and interpret genomic information. Although hybridization is fairly well understood in bulk solution, the greater complexity of an interfacial environment presents new challenges to a fundamental understanding, and hence application, of these assays. At a surface, molecular interactions are amplified by the two-dimensional nature of the immobilized layer, which focuses the nucleic acid charge and concentration to levels not encountered in solution, and which impacts the hybridization behavior in unique ways. This study finds that, at low ionic strengths, an electrostatic balance between the concentration of immobilized oligonucleotide charge and solution ionic strength governs the onset of hybridization. As ionic strength increases, the importance of electrostatics diminishes and the hybridization behavior becomes more complex. Suppression of hybridization affinity constants relative to solution values, and their weakened dependence on the concentration of DNA counterions, indicate that the immobilized strands form complexes that compete with hybridization to analyte strands. Moreover, an unusual regime is observed in which the surface coverage of immobilized oligonucleotides does not significantly influence the hybridization behavior, despite physical closeness and hence compulsory interactions between sites. These results are interpreted and summarized in a diagram of hybridization regimes that maps specific behaviors to experimental ranges of ionic strength and probe coverage.
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