ArticleThe Journal of biological chemistry2008
Functional surfaces on the p35/ARPC2 subunit of Arp2/3 complex required for cell growth, actin nucleation, and endocytosis.
Article in The Journal of biological chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 30 citations in OpenAlex.
- Increased airway epithelial cell-derived exosomes activate macrophage-mediated allergic inflammation via CD100 shedding.Journal of cellular and molecular medicine · 2021Article
- The Actin Cytoskeleton Mediates Transmission of "Applied and environmental microbiology · 2021Article
- ARPC2 promotes breast cancer proliferation and metastasis.Oncology reports · 2019Article
- Cortactin and fascin-1 regulate extracellular vesicle release by controlling endosomal trafficking or invadopodia formation and function.Scientific reports · 2018Article
- Mediator binds to boundaries of chromosomal interaction domains and to proteins involved in DNA looping, RNA metabolism, chromatin remodeling, and actin assembly.Nucleic acids research · 2017Article
- Arp2/3 complex controls T cell homeostasis by maintaining surface TCR levels via regulating TCRScientific reports · 2017Article
- Role of ARPC2 in Human Gastric Cancer.Mediators of inflammation · 2017Article
- The role of Arabidopsis Actin-Related Protein 3 in amyloplast sedimentation and polar auxin transport in root gravitropism.Journal of experimental botany · 2016Article
- Crucial roles of the Arp2/3 complex during mammalian corticogenesis.Development (Cambridge, England) · 2016Article
- Identification of an ATP-controlled allosteric switch that controls actin filament nucleation by Arp2/3 complex.Nature communications · 2016Article
- Actin and endocytosis in budding yeast.Genetics · 2015Review
- GMF severs actin-Arp2/3 complex branch junctions by a cofilin-like mechanism.Current biology : CB · 2013Article
- Insertions within the actin core of actin-related protein 3 (Arp3) modulate branching nucleation by Arp2/3 complex.The Journal of biological chemistry · 2013Article
- Purification of native Arp2/3 complex from bovine thymus.Methods in molecular biology (Clifton, N.J.) · 2013Article
- The ARP2/3 complex mediates guard cell actin reorganization and stomatal movement in Arabidopsis.The Plant cell · 2012Article
- Schizosaccharomyces pombe Arc3 is a conserved subunit of the Arp2/3 complex required for polarity, actin organization, and endocytosis.Yeast (Chichester, England) · 2011Article
- New mechanisms and functions of actin nucleation.Current opinion in cell biology · 2011Review
- An actin-filament-binding interface on the Arp2/3 complex is critical for nucleation and branch stability.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- The p40/ARPC1 subunit of Arp2/3 complex performs multiple essential roles in WASp-regulated actin nucleation.The Journal of biological chemistry · 2010Article
- Forward genetics in Candida albicans that reveals the Arp2/3 complex is required for hyphal formation, but not endocytosis.Molecular microbiology · 2010Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The Arp2/3 complex is comprised of seven evolutionarily conserved subunits and upon activation by WASp or another nucleation promoting factor nucleates the formation of actin filaments. These events are critical for driving a wide range of cellular processes, including motility, endocytosis, and intracellular trafficking. However, an in depth understanding of the Arp2/3 complex activation and nucleation mechanism is still lacking. Here, we used a mutagenesis approach in Saccharomyces cerevisiae to dissect the structural and functional roles of the p35/ARPC2 subunit. Using integrated alleles that target conserved and solvent-exposed residues, we identified surfaces on p35/ARPC2 required for cell growth, actin organization, and endocytosis. In parallel, we purified the mutant Arp2/3 complexes and compared their actin assembly activities both in the presence and in the absence of WASp. The majority of alleles with defects mapped to one face of p35/ARPC2, where there was a close correlation between loss of actin nucleation and endocytosis. A second site required for nucleation and endocytosis was identified near the contact surface between p35/ARPC2 and p19/ARPC4. A third site was identified at a more distal conserved surface, which was critical for endocytosis but not nucleation. These findings pinpoint the key surfaces on p35/ARPC2 required for Arp2/3 complex-mediated actin assembly and cellular function and provide a higher resolution view of Arp2/3 structure and mechanism.
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