ArticlePlant molecular biology2008
The maize Dof protein PBF activates transcription of gamma-zein during maize seed development.
Article in Plant molecular biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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36 citing papers in PubMed, 76 citations in OpenAlex.
- ZmDof08, a zinc finger transcription factor, plays critical roles in photosynthesis in maize.Plant cell reports · 2025Article
- Systematic Analysis ofInternational journal of molecular sciences · 2025Article
- Genome-wide identification and molecular evolution of Dof gene family in Camellia oleifera.BMC genomics · 2024Article
- Transcription factor ZmDof22 enhances drought tolerance by regulating stomatal movement and antioxidant enzymes activities in maize (Zea mays L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- Article
- Nitrogen-dependent binding of the transcription factor PBF1 contributes to the balance of protein and carbohydrate storage in maize endosperm.The Plant cell · 2023Article
- Genome-Wide Identification of DOF Gene Family and the Mechanism Dissection ofInternational journal of molecular sciences · 2022Article
- Ectopic Expression ofInternational journal of molecular sciences · 2022Article
- Underlying Biochemical and Molecular Mechanisms for Seed Germination.International journal of molecular sciences · 2022Review
- Article
- Regulators of Starch Biosynthesis in Cereal Crops.Molecules (Basel, Switzerland) · 2021Review
- The MYB family transcription factor TuODORANT1 from Triticum urartu and the homolog TaODORANT1 from Triticum aestivum inhibit seed storage protein synthesis in wheat.Plant biotechnology journal · 2021Article
- A novel NAC family transcription factor SPR suppresses seed storage protein synthesis in wheat.Plant biotechnology journal · 2021Article
- Proteome and Nutritional Shifts Observed in Hordein Double-Mutant Barley Lines.Frontiers in plant science · 2021Article
- The DOF-Domain Transcription Factor ZmDOF36 Positively Regulates Starch Synthesis in Transgenic Maize.Frontiers in plant science · 2019Article
- Transcriptome Analysis Identifies a Zinc Finger Protein Regulating Starch Degradation in Kiwifruit.Plant physiology · 2018Article
- GCTTCA as a novel motif for regulating mesocarp-specific expression of the oil palm (Elaeis guineensis Jacq.) stearoyl-ACP desaturase gene.Plant cell reports · 2018Article
- Identification and characterization of finger millet3 Biotech · 2018Article
- Identification and molecular characterization of Dof transcription factor gene family preferentially expressed in developing spikes of3 Biotech · 2018Article
- ZmDof3, a maize endosperm-specific Dof protein gene, regulates starch accumulation and aleurone development in maize endosperm.Plant molecular biology · 2017Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Maize PBF (prolamin-box binding factor) belongs to the Dof class of plant specific transcription factors containing one highly conserved zinc finger DNA-binding domain, called Dof (DNA binding with one finger) domain. Maize PBF trans-activates the gamma-zein gene (gammaZ) promoter in developing maize seeds as shown by transient expression in maize endosperms. Co-transfection of a gammaZ:GUS construct with 35S:PBF resulted in a sevenfold increase in GUS expression, however, PBF mutation in Cys residues within the Dof domain abolishes both, binding to DNA and the capacity to activate gammaZ promoter. We present two pieces of evidence that PBF transactivates gammaZ promoter by binding to the Pb3 motif (TGTAAAG). First, recombinant Dof domain of PBF (bdPBF) specifically recognized Pb3 site as shown by gel mobility shift assays and second, co-expression of PBF with gammaZ promoter mutated in Pb3 motif suppressed PBF trans-activation capacity. Immunocytochemical analysis on developing endosperm sections shows that PBF is localized in the nuclei of the peripheral layer cells of starchy endosperm, the tissue in which the initial accumulation of gamma-zein protein occurs. By contrast, PBF is detected in the cytosol of the starchy endosperm cells newly differentiated from aleurone daughter cells, where gamma-zein was absent. Taken together these data indicate that maize PBF plays an essential role in the regulation of the temporal and spatial expression of gammaZ gene.
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