ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2008
Microsatellites for the genus Cucurbita and an SSR-based genetic linkage map of Cucurbita pepo L.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Development of recombinant inbred lines and QTL analysis of plant height and fruit shape-related traits inMolecular breeding : new strategies in plant improvement · 2025Article
- Article
- Interspecific hybridization for transfer of hull-less seed trait from Cucurbita pepo to C. moschata.Scientific reports · 2023Article
- Genomic Position and Markers Associated with the Hull-Less Seed Trait in Pumpkin.Plants (Basel, Switzerland) · 2022Article
- Identification of single major QTL and candidate gene(s) governing hull-less seed trait in pumpkin.Frontiers in plant science · 2022Article
- A Novel QTL for Resistance to Phytophthora Crown Rot in Squash.Plants (Basel, Switzerland) · 2021Article
- Genetic Mapping and Identification of the Candidate Gene for White Seed Coat inInternational journal of molecular sciences · 2021Article
- Tracing back the origin of pumpkins (Cucurbita pepo ssp. pepo L.) in Mexico.Proceedings. Biological sciences · 2019Article
- Construction of a High-Density Genetic Map and Analysis of Seed-Related Traits Using Specific Length Amplified Fragment Sequencing forFrontiers in plant science · 2019Article
- Comparative analysis of microsatellite, SNP, and InDel markers in fourBreeding science · 2018Article
- A High-Density EST-SSR-Based Genetic Map and QTL Analysis of Dwarf Trait in Cucurbita pepo L.International journal of molecular sciences · 2018Article
- De novo assembly of the zucchini genome reveals a whole-genome duplication associated with the origin of the Cucurbita genus.Plant biotechnology journal · 2018Article
- Genetic mapping of ovary colour and quantitative trait loci for carotenoid content in the fruit ofMolecular breeding : new strategies in plant improvement · 2018Article
- Genetic Resources in the "Calabaza Pipiana" Squash (Frontiers in plant science · 2018Article
- A high-density linkage map and QTL mapping of fruit-related traits in pumpkin (Cucurbita moschata Duch.).Scientific reports · 2017Article
- An SNP-based saturated genetic map and QTL analysis of fruit-related traits in Zucchini using Genotyping-by-sequencing.BMC genomics · 2017Article
- Cultivar-Based Introgression Mapping Reveals Wild Species-Derived Pm-0, the Major Powdery Mildew Resistance Locus in Squash.PloS one · 2016Article
- A high-density genetic map for anchoring genome sequences and identifying QTLs associated with dwarf vine in pumpkin (Cucurbita maxima Duch.).BMC genomics · 2015Article
- Dynamics of sex expression and chromosome diversity in Cucurbitaceae: a story in the making.Journal of genetics · 2015Review
- An acorn squash (Cucurbita pepo ssp. ovifera) fruit and seed transcriptome as a resource for the study of fruit traits in Cucurbita.Horticulture research · 2015Article
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5 authors.
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Abstract
Until recently, only a few microsatellites have been available for Cucurbita, thus their development is highly desirable. The Austrian oil-pumpkin variety Gleisdorfer Olkürbis (C. pepo subsp. pepo) and the C. moschata cultivar Soler (Puerto Rico) were used for SSR development. SSR-enriched partial genomic libraries were established and 2,400 clones were sequenced. Of these 1,058 (44%) contained an SSR at least four repeats long. Primers were designed for 532 SSRs; 500 primer pairs produced fragments of expected size. Of these, 405 (81%) amplified polymorphic fragments in a set of 12 genotypes: three C. moschata, one C. ecuadorensis, and eight C. pepo representing all eight cultivar groups. On an average, C. pepo and C. moschata produced 3.3 alleles per primer pair, showing high inter-species transferability. There were 187 SSR markers detecting polymorphism between the USA oil-pumpkin variety "Lady Godiva" (O5) and the Italian crookneck variety "Bianco Friulano" (CN), which are the parents of our previous F(2) mapping population. It has been used to construct the first published C. pepo map, containing mainly RAPD and AFLP markers. Now the updated map comprises 178 SSRs, 244 AFLPs, 230 RAPDs, five SCARs, and two morphological traits (h and B). It contains 20 linkage groups with a map density of 2.9 cM. The observed genome coverage (Co) is 86.8%.
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