ArticleProceedings of the National Academy of Sciences of the United States of America2008
Blue light-induced autophosphorylation of phototropin is a primary step for signaling.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 108 papers.
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Who cites it
108 citing papers in PubMed, 263 citations in OpenAlex.
- Guard cell signal transduction mechanisms controlling stomatal movements by light, abscisic acid, CO2, VPD and heat.Plant physiology · 2026Review
- Blue light receptor phot2 collaborates with NRL-30 to negatively regulate immunity by reducing mitochondrial protein PRXIIF stability in potato.Molecular horticulture · 2026Article
- Dynamic and Basal Phosphorylation Landscapes of Abscisic Acid Signaling Revealed by Phosphoproteome Analysis in Arabidopsis.International journal of molecular sciences · 2026Article
- Phosphorylation of WDR48 by phototropins drives starch degradation to promote stomatal opening.Nature communications · 2026Article
- OmicIntegrator: A Simple and Versatile Tool for Meta-Analysis.Plants (Basel, Switzerland) · 2026Article
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- Reduced stomatal density in wheat overexpressing EPIDERMAL PATTERNING FACTOR1 differentially affects red and blue light responses.Plant physiology · 2025Article
- Histone deacetylase 9 modulates the acetylation dynamics of phototropin 1 to fine-tune phototropic responses in plants.Plant communications · 2025Article
- Identification of a novel stomatal opening chemical, PP242, that inhibits early abscisic acid signal transduction in guard cells.Plant & cell physiology · 2025Article
- Starch metabolism in guard cells: At the intersection of environmental stimuli and stomatal movement.Plant physiology · 2024Review
- Fungal toxin fusicoccin enhances plant growth by upregulating 14-3-3 interaction with plasma membrane HScientific reports · 2024Article
- Light-induced stomatal opening requires phosphorylation of the C-terminal autoinhibitory domain of plasma membrane HNature communications · 2024Article
- Arabidopsis COP1 guides stomatal response in guard cells through pH regulation.Communications biology · 2024Article
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- Paradise by the far-red light: Far-red and red:blue ratios independently affect yield, pigments, and carbohydrate production in lettuce,Frontiers in plant science · 2024Article
- Phototropin phosphorylation of ROOT PHOTOTROPISM 2 and its role in mediating phototropism, leaf positioning, and chloroplast accumulation movement in Arabidopsis.The Plant journal : for cell and molecular biology · 2023Article
- Moonlight Is Perceived as a Signal Promoting Genome Reorganization, Changes in Protein and Metabolite Profiles and Plant Growth.Plants (Basel, Switzerland) · 2023Article
- A comparison of stomatal conductance responses to blue and red light between C3 and C4 photosynthetic species in three phylogenetically-controlled experiments.Frontiers in plant science · 2023Article
- Machine Learning-Based Crop Stress Detection in Greenhouses.Plants (Basel, Switzerland) · 2022Article
48 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phototropins are autophosphorylating protein kinases of plant-specific blue light receptors. They regulate various blue light responses, including phototropism, chloroplast movements, hypocotyl growth inhibition, leaf flattening, and stomatal opening. However, the physiological role of autophosphorylation remains unknown. Here, we identified phosphorylation sites of Ser or Thr in the N terminus, Hinge1 region, kinase domain, and C terminus in Arabidopsis phototropin1 (phot1) by liquid chromatography-tandem mass spectrometry in vivo. We substituted these Ser or Thr residues with Ala in phot1 and analyzed their functions by inspecting the phot1-mediated responses of stomatal opening, phototropism, chloroplast accumulation, and leaf flattening after the transformation of the phot1 phot2 double mutant. Among these sites, we found that autophosphorylation of Ser-851 in the activation loop of the kinase domain was required for the responses mentioned above, whereas the phosphorylation of the other Ser and Thr, except those in the activation loop, was not. Ser-849 in the loop may have an additional role in the responses. Immunological analysis revealed that Ser-851 was phosphorylated rapidly by blue light in a fluence-dependent manner and dephosphorylated gradually upon darkness. We conclude that autophosphorylation of Ser-851 is a primary step that mediates signaling between photochemical reaction and physiological events.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.