ArticleThe Journal of cell biology2008
IP3 sensitizes TRPV4 channel to the mechano- and osmotransducing messenger 5'-6'-epoxyeicosatrienoic acid.
Article in The Journal of cell biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
61 citing papers in PubMed, 1 synthesis or guideline pooled it, 147 citations in OpenAlex.
- TRP Channels Interactome as a Novel Therapeutic Target in Breast Cancer.Frontiers in oncology · 2021Pooled it
- TRP Channels from Sensory Coding to Physiology.Metabolites · 2025Review
- A highly conserved neuronal microexon in DAAM1 controls actin dynamics, RHOA/ROCK signaling, and memory formation.Nature communications · 2025Article
- The Hunt for the Putative Epoxyeicosatrienoic Acid Receptor.ACS chemical biology · 2025Review
- Functions of TRPs in retinal tissue in physiological and pathological conditions.Frontiers in molecular neuroscience · 2024Review
- TRP Channels: Recent Development in Translational Research and Potential Therapeutic Targets in Migraine.International journal of molecular sciences · 2022Review
- Structural determinants of TRPV4 inhibition and identification of new antagonists with antiviral activity.British journal of pharmacology · 2022Article
- The mechanosensitive Piezo1 channel controls endosome trafficking for an efficient cytokinetic abscission.Science advances · 2021Article
- Angiotensin II Disrupts Neurovascular Coupling by Potentiating Calcium Increases in Astrocytic Endfeet.Journal of the American Heart Association · 2021Article
- TRPV4 receptor as a functional sensory molecule in bladder urothelium: Stretch-independent, tissue-specific actions and pathological implications.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2020Article
- Expression and Functional Role of TRPV4 in Bone Marrow-Derived CD11cInternational journal of molecular sciences · 2019Article
- Cell sensing and decision-making in confinement: The role of TRPM7 in a tug of war between hydraulic pressure and cross-sectional area.Science advances · 2019Article
- Magnetic Entropy as a Proposed Gating Mechanism for Magnetogenetic Ion Channels.Biophysical journal · 2019Article
- Sodium channel TRPM4 and sodium/calcium exchangers (NCX) cooperate in the control of CaThe Journal of biological chemistry · 2019Article
- TRPV4 Complexes With the NaFrontiers in physiology · 2019Article
- Construction of a Suite of Computable Biological Network Models Focused on Mucociliary Clearance in the Respiratory Tract.Frontiers in genetics · 2019Article
- Neurovascular dysfunction in vascular dementia, Alzheimer's and atherosclerosis.BMC neuroscience · 2018Review
- KChIP3 coupled to CaeLife · 2018Article
- The TRPV4 channel links calcium influx to DDX3X activity and viral infectivity.Nature communications · 2018Article
- TRPV4 (Transient Receptor Potential Vanilloid 4) Channel-Dependent Negative Feedback Mechanism Regulates GArteriosclerosis, thrombosis, and vascular biology · 2018Article
1 more citing papers are in PubMed but not listed here.
Corrections and comments
- Reprinted as
Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mechanical and osmotic sensitivity of the transient receptor potential vanilloid 4 (TRPV4) channel depends on phospholipase A(2) (PLA(2)) activation and the subsequent production of the arachidonic acid metabolites, epoxyeicosatrienoic acid (EET). We show that both high viscous loading and hypotonicity stimuli in native ciliated epithelial cells use PLA(2)-EET as the primary pathway to activate TRPV4. Under conditions of low PLA(2) activation, both also use extracellular ATP-mediated activation of phospholipase C (PLC)-inositol trisphosphate (IP(3)) signaling to support TRPV4 gating. IP(3), without being an agonist itself, sensitizes TRPV4 to EET in epithelial ciliated cells and cells heterologously expressing TRPV4, an effect inhibited by the IP(3) receptor antagonist xestospongin C. Coimmunoprecipitation assays indicated a physical interaction between TRPV4 and IP(3) receptor 3. Collectively, our study suggests a functional coupling between plasma membrane TRPV4 channels and intracellular store Ca(2+) channels required to initiate and maintain the oscillatory Ca(2+) signal triggered by high viscosity and hypotonic stimuli that do not reach a threshold level of PLA(2) activation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.