ArticleApplied and environmental microbiology2008
Evaluation of the persistence of infectious human noroviruses on food surfaces by using real-time nucleic acid sequence-based amplification.
Article in Applied and environmental microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 127 citations in OpenAlex.
- How Long Do Microorganisms Survive and Persist in Food? A Systematic Review.Microorganisms · 2025Review
- Tulane Virus Persistence and Microbial Stability in 3D Food Ink under Various Storage Conditions: A Pre- and Post-Printing Analysis.Food and environmental virology · 2024Article
- Investigating the microbial inactivation effect of low temperature high pressure carbon dioxide and its application in frozen prawn (Food control · 2023Article
- Discrimination and surveillance of infectious severe acute respiratory syndrome Coronavirus 2 in wastewater using cell culture and RT-qPCR.The Science of the total environment · 2022Article
- Persistence of Hepatitis A Virus RNA in Water, on Non-porous Surfaces, and on Blueberries.Frontiers in microbiology · 2021Article
- A Survey of Analytical Techniques for Noroviruses.Foods (Basel, Switzerland) · 2020Review
- Survival and inactivation of human norovirus GII.4 Sydney on commonly touched airplane cabin surfaces.AIMS public health · 2020Article
- Comparison of Swab Sampling Methods for Norovirus Recovery on Surfaces.Food and environmental virology · 2018Article
- Viability RT-qPCR to Distinguish Between HEV and HAV With Intact and Altered Capsids.Frontiers in microbiology · 2018Article
- Detection of Potential Infectious Enteric Viruses in Fresh Produce by (RT)-qPCR Preceded by Nuclease Treatment.Food and environmental virology · 2017Article
- Recovery Optimization and Survival of the Human Norovirus Surrogates Feline Calicivirus and Murine Norovirus on Carpet.Applied and environmental microbiology · 2017Article
- Non-heat-treated frozen raspberries the most likely vehicle of a norovirus outbreak in Oslo, Norway, November 2013.Epidemiology and infection · 2016Article
- Identification of Enteric Viruses in Foods from Mexico City.Food and environmental virology · 2016Article
- Inactivation Kinetics and Mechanism of a Human Norovirus Surrogate on Stainless Steel Coupons via Chlorine Dioxide Gas.Applied and environmental microbiology · 2016Article
- Abiotic Stress and Phyllosphere Bacteria Influence the Survival of Human Norovirus and Its Surrogates on Preharvest Leafy Greens.Applied and environmental microbiology · 2016Article
- Enzymatic Pre-treatment of Wastewater to Minimize Recovery by Reverse Transcriptase PCR of RNA from Inactive Bacteriophages.Current microbiology · 2015Article
- Comparison of methods for evaluating the thermal stability of human enteric viruses.Food and environmental virology · 2015Review
- Persistence of human norovirus RT-qPCR signals in simulated gastric fluid.Food and environmental virology · 2015Article
- Norovirus transmission between hands, gloves, utensils, and fresh produce during simulated food handling.Applied and environmental microbiology · 2014Article
- Infection control for norovirus.Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases · 2014Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Noroviruses (NoV) are the major cause of nonbacterial gastroenteritis. However, there is no published study to ascertain their survival on foodstuffs which are directly related to human health risk. In the present study, we developed a rapid, simple, and sensitive real-time nucleic acid sequence-based amplification (NASBA) combined with an enzymatic treatment for distinguishing infectious from noninfectious human NoV. The developed method was validated using spiked ready-to-eat food samples. When feline calicivirus (FCV) was used as a NoV surrogate in the preliminary assays, it appeared more sensitive to heat inactivation and enzymatic pretreatment than the human NoV. This suggests that FCV may not be an ideal model for studying NoV. Our results reveal clearly that the developed enzymatic pretreatment/real-time NASBA combination successfully distinguished the infectious from heat-inactivated NoV. Moreover, we demonstrate that NoV survived for at least 10 days on refrigerated ready-to-eat foods, such as lettuce and turkey. However, the survival rate was higher on turkey than on lettuce, probably because of their different surface natures. The approach developed in this study may be suitable for more in-depth studies of the persistence and inactivation of human NoV and may be applied to other nonculturable RNA viruses. Moreover, the evaluation of infectious NoV survival provided valuable information concerning its persistence on ready-to-eat food.
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