ArticleJournal of clinical virology : the official publication of the Pan American Society for Clinical Virology2008
Development of real-time PCR assays for detection and quantification of human bocavirus.
Article in Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
11 citing papers in PubMed, 25 citations in OpenAlex.
- Detection of Feline Coronavirus Membrane Gene Based on Conventional Revere Transcription-Polymerase Chain Reaction, Nested Reverse Transcription-Polymerase Chain Reaction, and Reverse Transcription-Quantitative Polymerase Chain Reaction: A Comparative Study.International journal of molecular sciences · 2025Article
- Microfluidic-based virus detection methods for respiratory diseases.Emergent materials · 2021Review
- [Detection and analysis of bocavirus in hospitalized children with respiratory infection].Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics · 2016Article
- Seroepidemiology of human bocaviruses 1 and 2 in China.PloS one · 2015Article
- Prevalence and molecular epidemiology of human coronavirus HKU1 in patients with acute respiratory illness.Journal of medical virology · 2013Article
- Epidemic viral pneumonia and other emerging pathogens.Clinics in chest medicine · 2011Review
- Molecular diagnosis of viral respiratory infections.Current infectious disease reports · 2011Article
- Real-time detection of influenza a, influenza B, and respiratory syncytial virus a and B in respiratory specimens by use of nanoparticle probes.Journal of clinical microbiology · 2010Article
- Hematopoietic cell transplantation and emerging viral infections.Journal of medical virology · 2010Review
- Evidence of prior exposure to human bocavirus as determined by a retrospective serological study of 404 serum samples from adults in the United States.Clinical and vaccine immunology : CVI · 2009Article
- Clinical and epidemiological aspects of human bocavirus infection.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2008Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundHuman bocavirus (HBoV) is a parvovirus that has been recently detected in patients with respiratory illness.
objectivesWe developed a sensitive, specific, and quantitative real-time PCR assay based on the TaqMan method for HBoV detection and quantification in respiratory specimens. STUDY
designThree individual real-time PCR assays were designed to amplify HBoV NS1, NP-1, and VP1 genes. For clinical evaluation, 506 nasal aspirates obtained from patients with acute respiratory tract infections during December 2006 to May 2007 were tested.
resultsEach assay had a broad dynamic range (50 x 10(7) to 5 x 10(7)copies of plasmid DNA) and high inter- and intra-assay reproducibility. The detection limit of each assay was 10 genome copies per reaction, and no crossreactivity with other major respiratory viruses or bacteria was detected. Clinical evaluation revealed that 11 (2.1%) of 506 patients diagnosed with upper respiratory tract infections, pneumonia, bronchitis, pharyngitis, or sinusitis had HBoV detected by all three assays, with viral loads ranging from 8.2 x 10(4) to 8.1 x 10(9)copies/ml of specimen.
conclusionsThe three assays for HBoV diagnosis and quantification are highly sensitive, specific real-time tools for the reliable epidemiological and pathogenetic study of HBoV infection.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.