ArticleJournal of biosciences2008
A gene-trap strategy identifies quiescence-induced genes in synchronized myoblasts.
Article in Journal of biosciences, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 18 citations in OpenAlex.
- Mimicking Muscle Stem Cell Quiescence in Culture: Methods for Synchronization in Reversible Arrest.Methods in molecular biology (Clifton, N.J.) · 2017Article
- Impact of MLL5 expression on decitabine efficacy and DNA methylation in acute myeloid leukemia.Haematologica · 2014Article
- A gene trap mutagenesis screen for genes underlying cellular response to the mood stabilizer lithium.Journal of cellular and molecular medicine · 2013Article
- A novel in vitro model for studying quiescence and activation of primary isolated human myoblasts.PloS one · 2013Article
- Epigenetic regulation of planarian stem cells by the SET1/MLL family of histone methyltransferases.Epigenetics · 2013Article
- Expression and roles of NUPR1 in cholangiocarcinoma cells.Anatomy & cell biology · 2012Article
- Trithorax group proteins: switching genes on and keeping them active.Nature reviews. Molecular cell biology · 2011Review
- Metabolic status rather than cell cycle signals control quiescence entry and exit.The Journal of cell biology · 2011Article
- Drosophila SIN3 isoforms interact with distinct proteins and have unique biological functions.The Journal of biological chemistry · 2010Article
- Phosphorylation of mixed lineage leukemia 5 by CDC2 affects its cellular distribution and is required for mitotic entry.The Journal of biological chemistry · 2010Article
- Stress-inducible protein p8 is involved in several physiological and pathological processes.The Journal of biological chemistry · 2010Review
- The small chromatin-binding protein p8 coordinates the association of anti-proliferative and pro-myogenic proteins at the myogenin promoter.Journal of cell science · 2009Article
- MLL5, a trithorax homolog, indirectly regulates H3K4 methylation, represses cyclin A2 expression, and promotes myogenic differentiation.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
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Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cellular quiescence is characterized not only by reduced mitotic and metabolic activity but also by altered gene expression. Growing evidence suggests that quiescence is not merely a basal state but is regulated by active mechanisms. To understand the molecular programme that governs reversible cell cycle exit, we focused on quiescence-related gene expression in a culture model of myogenic cell arrest and activation. Here we report the identification of quiescence-induced genes using a gene-trap strategy. Using a retroviral vector, we generated a library of gene traps in C2C12 myoblasts that were screened for arrest-induced insertions by live cell sorting (FACS-gal). Several independent gene- trap lines revealed arrest-dependent induction of betagal activity, confirming the efficacy of the FACS screen. The locus of integration was identified in 15 lines. In three lines,insertion occurred in genes previously implicated in the control of quiescence, i.e. EMSY - a BRCA2--interacting protein, p8/com1 - a p300HAT -- binding protein and MLL5 - a SET domain protein. Our results demonstrate that expression of chromatin modulatory genes is induced in G0, providing support to the notion that this reversibly arrested state is actively regulated.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.