ArticleMicrobiology (Reading, England)2008
Identification of Haemophilus ducreyi genes expressed during human infection.
Article in Microbiology (Reading, England), 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 28 citations in OpenAlex.
- Molecular pathogenesis ofMicrobiology and molecular biology reviews : MMBR · 2025Review
- Article
- Infections caused byClinical microbiology reviews · 2024Review
- Transmission of viable Haemophilus ducreyi by Musca domestica.PLoS neglected tropical diseases · 2024Article
- Article
- Putative vaccine candidates and drug targets identified by reverse vaccinology and subtractive genomics approaches to controlJournal of the Royal Society, Interface · 2018Article
- Transcriptomics in Human Challenge Models.Frontiers in immunology · 2017Review
- Insights into Campylobacter jejuni colonization and enteritis using a novel infant rabbit model.Scientific reports · 2016Article
- Haemophilus ducreyi Seeks Alternative Carbon Sources and Adapts to Nutrient Stress and Anaerobiosis during Experimental Infection of Human Volunteers.Infection and immunity · 2016Article
- A (p)ppGpp-null mutant of Haemophilus ducreyi is partially attenuated in humans due to multiple conflicting phenotypes.Infection and immunity · 2014Article
- Outer membrane protein P4 is not required for virulence in the human challenge model of Haemophilus ducreyi infection.BMC microbiology · 2014Article
- The Haemophilus ducreyi Fis protein is involved in controlling expression of the lspB-lspA2 operon and other virulence factors.Infection and immunity · 2013Article
- Expression of the Flp proteins by Haemophilus ducreyi is necessary for virulence in human volunteers.BMC microbiology · 2011Article
- Haemophilus ducreyi lipooligosaccharides induce expression of the immunosuppressive enzyme indoleamine 2,3-dioxygenase via type I interferons and tumor necrosis factor alpha in human dendritic cells.Infection and immunity · 2011Article
- A Haemophilus ducreyi CpxR deletion mutant is virulent in human volunteers.The Journal of infectious diseases · 2011Article
- Analysis of Salmonella enterica serotype paratyphi A gene expression in the blood of bacteremic patients in Bangladesh.PLoS neglected tropical diseases · 2010Article
- Activation of the CpxRA system by deletion of cpxA impairs the ability of Haemophilus ducreyi to infect humans.Infection and immunity · 2010Article
- Haemophilus ducreyi SapA contributes to cathelicidin resistance and virulence in humans.Infection and immunity · 2010Article
- Host-pathogen interplay of Haemophilus ducreyi.Current opinion in infectious diseases · 2010Review
- Regulation of expression of the Haemophilus ducreyi LspB and LspA2 proteins by CpxR.Infection and immunity · 2009Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
To identify Haemophilus ducreyi transcripts that are expressed during human infection, we used selective capture of transcribed sequences (SCOTS) with RNA isolated from pustules obtained from three volunteers infected with H. ducreyi, and with RNA isolated from broth-grown bacteria used to infect volunteers. With SCOTS, competitive hybridization of tissue-derived and broth-derived sequences identifies genes that may be preferentially expressed in vivo. Among the three tissue specimens, we identified 531 genes expressed in vivo. Southern blot analysis of 60 genes from each tissue showed that 87 % of the identified genes hybridized better with cDNA derived from tissue specimens than with cDNA derived from broth-grown bacteria. RT-PCR on nine additional pustules confirmed in vivo expression of 10 of 11 selected genes in other volunteers. Of the 531 genes, 139 were identified in at least two volunteers. These 139 genes fell into several functional categories, including biosynthesis and metabolism, regulation, and cellular processes, such as transcription, translation, cell division, DNA replication and repair, and transport. Detection of genes involved in anaerobic and aerobic respiration indicated that H. ducreyi likely encounters both microenvironments within the pustule. Other genes detected suggest an increase in DNA damage and stress in vivo. Genes involved in virulence in other bacterial pathogens and 32 genes encoding hypothetical proteins were identified, and may represent novel virulence factors. We identified three genes, lspA1, lspA2 and tadA, known to be required for virulence in humans. This is the first study to broadly define transcripts expressed by H. ducreyi in humans.
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Registered trials
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