ArticleEukaryotic cell2008
Overlapping functions of the two talin homologues in Dictyostelium.
Article in Eukaryotic cell, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 42 citations in OpenAlex.
- Talin force coupling underlies eukaryotic cell-substrate adhesion.Nature communications · 2025Article
- Reduced cell-substrate adhesion formation promotes cell migration inMolecular biology of the cell · 2025Article
- Filopodia numbers impact chemotactic migration speed.bioRxiv : the preprint server for biology · 2025Article
- Reduced cell-substrate adhesion formation promotes cell migration inbioRxiv : the preprint server for biology · 2025Article
- A negative feedback loop between small GTPase Rap1 and mammalian tumor suppressor homologue KrsB regulates cell-substrate adhesion inMolecular biology of the cell · 2025Article
- The IQGAP-related RasGAP IqgC regulates cell-substratum adhesion in Dictyostelium discoideum.Cellular & molecular biology letters · 2025Article
- Gradients of PI(4,5)PFrontiers in cell and developmental biology · 2022Article
- An evolutionary and physiological perspective on cell-substrate adhesion machinery for cell migration.Frontiers in cell and developmental biology · 2022Review
- Adhesion ofFrontiers in cell and developmental biology · 2022Review
- Role of LrrkA in the Control of Phagocytosis and Cell Motility inFrontiers in cell and developmental biology · 2021Article
- Cell-substrate adhesion drives Scar/WAVE activation and phosphorylation by a Ste20-family kinase, which controls pseudopod lifetime.PLoS biology · 2020Article
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- High-throughput Measurement of Dictyostelium discoideum Macropinocytosis by Flow Cytometry.Journal of visualized experiments : JoVE · 2018Article
- Glycogen synthase kinase 3 promotes multicellular development over unicellular encystation in encysting Dictyostelia.EvoDevo · 2018Article
- Loss of FrmB results in increased size of developmental structures during the multicellular development of Dictyostelium cells.Journal of microbiology (Seoul, Korea) · 2017Article
- Mechanosensitive Adhesion Explains Stepping Motility in Amoeboid Cells.Biophysical journal · 2017Article
- Direct Interaction between TalinB and Rap1 is necessary for adhesion of Dictyostelium cells.BMC cell biology · 2016Article
- Secreted Cyclic Di-GMP Induces Stalk Cell Differentiation in the Eukaryote Dictyostelium discoideum.Journal of bacteriology · 2016Review
- Moving towards a paradigm: common mechanisms of chemotactic signaling in Dictyostelium and mammalian leukocytes.Cellular and molecular life sciences : CMLS · 2014Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Talin is a cytoskeletal protein involved in constructing and regulating focal adhesions in animal cells. The cellular slime mold Dictyostelium discoideum has two talin homologues, talA and talB, and earlier studies have characterized the single knockout mutants. talA(-) cells show reduced adhesion to the substrates and slightly impaired cytokinesis leading to a high proportion of multinucleated cells in the vegetative stage, while the development is normal. In contrast, talB(-) cells are characterized by reduced motility in the developmental stage, and they are arrested at the tight-mound stage. Here, we created and analyzed a double mutant with a disruption of both talA and talB. Defects in adhesion to the substrates, cytokinesis, and development were more severe in cells with a disruption of both talA and talB. The talA(-) talB(-) cells failed to attach to the substrates in the vegetative stage, exhibited a higher proportion of multinucleated cells than talA(-) cells, and showed more-reduced motility during the development and an earlier developmental arrest than talB(-) cells at the loose-mound stage. Moreover, overexpression of either talA or talB compensated for the loss of the other talin, respectively. The analysis of talA(-) talB(-) cells also revealed that talin was required for the formation of paxillin-rich adhesion sites and that there was another adhesion mechanism which is independent of talin in the developmental stage. This is the first study demonstrating overlapping functions of two talin homologues, and our data further indicate the importance of talin.
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