ArticleJournal of bacteriology2008
Transcriptional regulation of the tad locus in Aggregatibacter actinomycetemcomitans: a termination cascade.
Article in Journal of bacteriology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Type IV pilin regulation: a transcriptional overview.Critical reviews in microbiology · 2026Review
- Review
- The Trimeric Autotransporter Adhesin EmaA and Infective Endocarditis.Pathogens (Basel, Switzerland) · 2024Review
- Regulation of adhesin synthesis in Aggregatibacter actinomycetemcomitans.Molecular oral microbiology · 2023Article
- Differential Virulence of Aggregatibacter actinomycetemcomitans Serotypes Explained by Exoproteome Heterogeneity.Microbiology spectrum · 2023Article
- Complete Genome Sequence of Aggregatibacter actinomycetemcomitans Strain CU1000N.Microbiology resource announcements · 2022Article
- Transcriptome profiling ofAccess microbiology · 2020Article
- Article
- The cell envelope proteome of Aggregatibacter actinomycetemcomitans.Molecular oral microbiology · 2015Article
- Role and regulation of the Flp/Tad pilus in the virulence of Pectobacterium atrosepticum SCRI1043 and Pectobacterium wasabiae SCC3193.PloS one · 2013Article
- The product of tadZ, a new member of the parA/minD superfamily, localizes to a pole in Aggregatibacter actinomycetemcomitans.Molecular microbiology · 2012Article
- Transcriptional activation of the tad type IVb pilus operon by PypB in Yersinia enterocolitica.Journal of bacteriology · 2010Article
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4 authors.
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Abstract
The tad (tight adherence) locus of Aggregatibacter actinomycetemcomitans includes genes for the biogenesis of Flp pili, which are necessary for bacterial adhesion to surfaces, biofilm formation, and pathogenesis. Although studies have elucidated the functions of some of the Tad proteins, little is known about the regulation of the tad locus in A. actinomycetemcomitans. A promoter upstream of the tad locus was previously identified and shown to function in Escherichia coli. Using a specially constructed reporter plasmid, we show here that this promoter (tadp) functions in A. actinomycetemcomitans. To study expression of the pilin gene (flp-1) relative to that of tad secretion complex genes, we used Northern hybridization analysis and a lacZ reporter assay. We identified three terminators, two of which (T1 and T2) can explain flp-1 mRNA abundance, while the third (T3) is at the end of the locus. T1 and T3 have the appearance and behavior of intrinsic terminators, while T2 has a different structure and is inhibited by bicyclomycin, indicating that T2 is probably Rho dependent. To help achieve the appropriate stoichiometry of the Tad proteins, we show that a transcriptional-termination cascade is important to the proper expression of the tad genes. These data indicate a previously unreported mechanism of regulation in A. actinomycetemcomitans and lead to a more complete understanding of its Flp pilus biogenesis.
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