ArticleJournal of bacteriology2008
Genetic analysis of the methanol- and methylamine-specific methyltransferase 2 genes of Methanosarcina acetivorans C2A.
Article in Journal of bacteriology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 43 citations in OpenAlex.
- Pyrrolysine supply and demand shapes the translational landscape inProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Methanol-specific methyltransferase isozymes have large carbon kinetic isotope effects that impact methane isotopic signatures.Applied and environmental microbiology · 2026Article
- An integrated culturomic and genomic database and analysis platform for methanogenic archaea.Database : the journal of biological databases and curation · 2026Article
- The Clumped Isotope Signatures of Multiple Methanogenic Metabolisms.Environmental science & technology · 2025Article
- Mer overexpression inApplied and environmental microbiology · 2025Article
- Pyruvate-dependent growth ofJournal of bacteriology · 2024Article
- Supplementation of Sulfide or Acetate and 2-Mercaptoethane Sulfonate Restores Growth of theMicroorganisms · 2023Article
- Energy Conservation and Hydrogenase Function in Methanogenic Archaea, in Particular the GenusMicrobiology and molecular biology reviews : MMBR · 2019Review
- A Membrane-Bound Cytochrome EnablesmBio · 2019Article
- Biochemical Characterization of the Methylmercaptopropionate:Cob(I)alamin Methyltransferase from Methanosarcina acetivorans.Journal of bacteriology · 2019Article
- Article
- Genome-Scale Metabolic Modeling of Archaea Lends Insight into Diversity of Metabolic Function.Archaea (Vancouver, B.C.) · 2017Review
- Growth Characteristics ofArchaea (Vancouver, B.C.) · 2017Article
- Assessing methanotrophy and carbon fixation for biofuel production by Methanosarcina acetivorans.Microbial cell factories · 2016Article
- Rerouting Cellular Electron Flux To Increase the Rate of Biological Methane Production.Applied and environmental microbiology · 2015Article
- Radical S-adenosylmethionine enzymes.Chemical reviews · 2014Review
- Genetic resources for methane production from biomass described with the Gene Ontology.Frontiers in microbiology · 2014Article
- Phylogenomic data support a seventh order of Methylotrophic methanogens and provide insights into the evolution of Methanogenesis.Genome biology and evolution · 2013Article
- An archaeal sRNA targeting cis- and trans-encoded mRNAs via two distinct domains.Nucleic acids research · 2012Article
- Functional context, biosynthesis, and genetic encoding of pyrrolysine.Current opinion in microbiology · 2011Review
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The entry of methanol into the methylotrophic pathway of methanogenesis is mediated by the concerted effort of two methyltransferases, namely, methyltransferase 1 (MT1) and methyltransferase 2 (MT2). The mtaA1, mtaA2, and mtbA genes of Methanosarcina acetivorans C2A encode putative methanol- or methylamine-specific MT2 enzymes. To address the in vivo roles of these genes in growth and methanogenesis from known substrates, we constructed and characterized mutants with deletions of each of these genes. The mtaA1 gene is required for growth on methanol, whereas mtaA2 was dispensable. However, the mtaA2 mutant had a reduced rate of methane production from methanol. Surprisingly, deletion of mtaA1 in combination with deletions of the genes encoding three methanol-specific MT1 isozymes led to lack of growth on acetate, suggesting that MT1 and MT2 enzymes might play an important role during growth on this substrate. The mtbA gene was required for dimethylamine and monomethylamine (MMA) utilization and was important, but not required, for trimethylamine utilization. Analysis of reporter gene fusions revealed that both mtaA1 and mtbA were expressed on all methanogenic substrates tested. However, mtaA1 expression was induced on methanol, while mtbA expression was down-regulated on MMA and acetate. mtaA2 was expressed at very low levels on all substrates. The mtaA1 transcript had a large 5' untranslated region (UTR) (275 bp), while the 5' UTR of the mtbA transcript was only 28 bp long.
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