ArticleBiophysical journal2008
Real-time kinetic modeling of voltage-gated ion channels using dynamic clamp.
Article in Biophysical journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 92 citations in OpenAlex.
- Complex excitability and "flipping" of granule cells: An experimental and computational study.PloS one · 2026Article
- From Flux to Function: Extracting Mechanistic Insights from Ion Channels via I-V and I-μ Analyses.bioRxiv : the preprint server for biology · 2025Article
- Dynamic Clamp Methods to Investigate Impaired Neuronal Excitability Associated with Autism.Journal of visualized experiments : JoVE · 2025Article
- The background sodium leak channel NALCN is a major controlling factor in pituitary cell excitability.The Journal of physiology · 2025Article
- Long-term, Dynamic Remodelling of the Corticotroph Transcriptome and Excitability After a Period of Chronic Stress.Endocrinology · 2024Article
- α-cell electrophysiology and the regulation of glucagon secretion.The Journal of endocrinology · 2023Review
- Sex differences in pituitary corticotroph excitability.Frontiers in physiology · 2023Article
- Identification of structures for ion channel kinetic models.PLoS computational biology · 2021Article
- Calibration of ionic and cellular cardiac electrophysiology models.Wiley interdisciplinary reviews. Systems biology and medicine · 2020Review
- Ion channel noise shapes the electrical activity of endocrine cells.PLoS computational biology · 2020Article
- Article
- Four Ways to Fit an Ion Channel Model.Biophysical journal · 2019Article
- From Local to Global Modeling for Characterizing Calcium Dynamics and Their Effects on Electrical Activity and Exocytosis in Excitable Cells.International journal of molecular sciences · 2019Article
- Estradiol Enhances the Depolarizing Response to GABA and AMPA Synaptic Conductances in Arcuate Kisspeptin Neurons by Diminishing Voltage-Gated Potassium Currents.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2019Article
- Resurgent Na+ Current Offers Noise Modulation in Bursting Neurons.PLoS computational biology · 2019Article
- Changes in Both Neuron Intrinsic Properties and Neurotransmission Are Needed to Drive the Increase in GnRH Neuron Firing Rate during Estradiol-Positive Feedback.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2019Article
- Kinetic properties of persistent NaThe Journal of general physiology · 2018Article
- Estimating kinetic mechanisms with prior knowledge I: Linear parameter constraints.The Journal of general physiology · 2018Article
- Estimating kinetic mechanisms with prior knowledge II: Behavioral constraints and numerical tests.The Journal of general physiology · 2018Article
- A single Markov-type kinetic model accounting for the macroscopic currents of all human voltage-gated sodium channel isoforms.PLoS computational biology · 2017Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
We propose what to our knowledge is a new technique for modeling the kinetics of voltage-gated ion channels in a functional context, in neurons or other excitable cells. The principle is to pharmacologically block the studied channel type, and to functionally replace it with dynamic clamp, on the basis of a computational model. Then, the parameters of the model are modified in real time (manually or automatically), with the objective of matching the dynamical behavior of the cell (e.g., action potential shape and spiking frequency), but also the transient and steady-state properties of the model (e.g., those derived from voltage-clamp recordings). Through this approach, one may find a model and parameter values that explain both the observed cellular dynamics and the biophysical properties of the channel. We extensively tested the method, focusing on Na(v) models. Complex Markov models (10-12 states or more) could be accurately integrated in real time at >50 kHz using the transition probability matrix, but not the explicit Euler method. The practicality of the technique was tested with experiments in raphe pacemaker neurons. Through automated real-time fitting, a Hodgkin-Huxley model could be found that reproduced well the action potential shape and the spiking frequency. Adding a virtual axonal compartment with a high density of Na(v) channels further improved the action potential shape. The computational procedure was implemented in the free QuB software, running under Microsoft Windows and featuring a friendly graphical user interface.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.