ArticleMolecular endocrinology (Baltimore, Md.)2008
Protein kinase Calpha-induced derepression of the human luteinizing hormone receptor gene transcription through ERK-mediated release of HDAC1/Sin3A repressor complex from Sp1 sites.
Article in Molecular endocrinology (Baltimore, Md.), 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 38 citations in OpenAlex.
- Synergistic activation of mutant TERT promoter by Sp1 and GABPA in BRAFNPJ precision oncology · 2021Article
- Molecular mechanism of FSHR expression induced by BMP15 in human granulosa cells.Journal of assisted reproduction and genetics · 2019Article
- Interaction of positive coactivator 4 with histone 3.3 protein is essential for transcriptional activation of the luteinizing hormone receptor gene.Biochimica et biophysica acta. Gene regulatory mechanisms · 2018Article
- The histone demethylase PHF8 is a molecular safeguard of the IFNγ response.Nucleic acids research · 2017Article
- Activation of mutant TERT promoter by RAS-ERK signaling is a key step in malignant progression of BRAF-mutant human melanomas.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Disruptions in follicle cell functions in the ovaries of rhesus monkeys during summer.Physiological genomics · 2015Article
- Glutamine contributes to maintenance of mouse embryonic stem cell self-renewal through PKC-dependent downregulation of HDAC1 and DNMT1/3a.Cell cycle (Georgetown, Tex.) · 2015Article
- Contribution of transcription factor, SP1, to the promotion of HB-EGF expression in defense mechanism against the treatment of irinotecan in ovarian clear cell carcinoma.Cancer medicine · 2014Article
- Lhcgr expression in granulosa cells: roles for PKA-phosphorylated β-catenin, TCF3, and FOXO1.Molecular endocrinology (Baltimore, Md.) · 2013Article
- The Justy mutant mouse strain produces a spontaneous murine model of salivary gland cancer with myoepithelial and basal cell differentiation.Laboratory investigation; a journal of technical methods and pathology · 2013Article
- Regulation of integrin αV subunit expression by sulfatide in hepatocellular carcinoma cells.Journal of lipid research · 2013Article
- Rsk2 Knockdown in PC12 Cells Results in Sp1 Dependent Increased Expression of the Gria2 Gene, Encoding the AMPA Receptor Subunit GluR2.International journal of molecular sciences · 2013Article
- Article
- Androgen-induced activation of gonadotropin-regulated testicular RNA helicase (GRTH/Ddx25) transcription: essential role of a nonclassical androgen response element half-site.Molecular and cellular biology · 2012Article
- PKC isoforms interact with and phosphorylate DNMT1.BMC biology · 2011Article
- Proinflammatory stimuli control N-acylphosphatidylethanolamine-specific phospholipase D expression in macrophages.Molecular pharmacology · 2011Article
- Coactivator function of positive cofactor 4 (PC4) in Sp1-directed luteinizing hormone receptor (LHR) gene transcription.The Journal of biological chemistry · 2011Article
- Ischemia/reperfusion reduces transcription factor Sp1-mediated cystathionine beta-synthase expression in the kidney.The Journal of biological chemistry · 2010Article
- Article
- Participation of signaling pathways in the derepression of luteinizing hormone receptor transcription.Molecular and cellular endocrinology · 2010Review
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
LH receptor (LHR) gene transcription is subject to repression/derepression through various modes and multiple effectors. Epigenetic silencing and activation of the LHR is achieved through coordinated regulation at both histone and DNA levels. The LHR gene is subject to repression by deacetylation and methylation at its promoter region, where a HDAC/mSin3A repressor complex is anchored at Sp1 sites. The present studies revealed that protein kinase C (PKC) alpha/ERK signaling is important for the activation of LHR promoter activity, and the increase of endogenous transcripts induced by phorbol-12-myristate-13-acetate (PMA) in HeLa cells. Whereas these effects were attributable to PKCalpha activity, the ERK pathway was the downstream effector in LHR activation. PMA caused a significant enhancement of Sp1 phosphorylation at serine residue (s), which was blocked by PKCalpha or ERK inhibition. The interaction of activated phosphorylated ERK with Sp1 and ERK's association with the LHR promoter points to Sp1 as a direct target of ERK. After Sp1 phosphorylation, the HDAC1/mSin3A repressor complex dissociated from Sp1 sites, histone 3 was acetylated, and transcription factor II B and RNA polymerase II were recruited. In addition, overexpression of a constitutively active PKCalpha (PKCalpha CA) strongly activated LHR transcription in MCF-7 cells (devoid of PKCalpha), induced Sp1 phosphorylation at serine residue (s) and caused derecruitment of HDAC1/mSin3A complex from the promoter. These effects were negated by cotransfection of a dominant-negative PKCalpha. In conclusion, these studies have revealed a novel regulatory signaling mechanism of transcriptional control in which the LHR is derepressed through PKCalpha/ERK-mediated Sp1 phosphorylation, causing the release of HDAC1/mSin3A complex from the promoter.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.