ArticleThe Journal of physiology2008
A technique for simultaneous measurement of Ca2+, FRET fluorescence and force in intact mouse small arteries.
Article in The Journal of physiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 21 citations in OpenAlex.
- Mathematical modeling and biochemical analysis support partially ordered calmodulin-myosin light chain kinase binding.iScience · 2023Article
- Imaging sympathetic neurogenic CaAutonomic neuroscience : basic & clinical · 2017Review
- Regulation of Glucokinase by Intracellular Calcium Levels in Pancreatic β Cells.The Journal of biological chemistry · 2016Article
- PC-PLC/sphingomyelin synthase activity plays a central role in the development of myogenic tone in murine resistance arteries.American journal of physiology. Heart and circulatory physiology · 2015Article
- Cytoskeletal reorganization evoked by Rho-associated kinase- and protein kinase C-catalyzed phosphorylation of cofilin and heat shock protein 27, respectively, contributes to myogenic constriction of rat cerebral arteries.The Journal of biological chemistry · 2014Article
- A method for noninvasive longitudinal measurements of [Ca2+] in arterioles of hypertensive optical biosensor mice.American journal of physiology. Heart and circulatory physiology · 2014Article
- Imaging intraorganellar Ca2+ at subcellular resolution using CEPIA.Nature communications · 2014Article
- Ca(2+) signaling in arterioles and small arteries of conscious, restrained, optical biosensor mice.Frontiers in physiology · 2014Article
- Intravital Förster resonance energy transfer imaging reveals elevated [Ca2+]i and enhanced sympathetic tone in femoral arteries of angiotensin II-infused hypertensive biosensor mice.The Journal of physiology · 2013Article
- Vascular tone and Ca(2+) signaling in murine cremaster muscle arterioles in vivo.Microcirculation (New York, N.Y. : 1994) · 2013Article
- Article
- Imaging the coordination of multiple signalling activities in living cells.Nature reviews. Molecular cell biology · 2011Review
- Design and application of genetically encoded biosensors.Trends in biotechnology · 2011Review
- In vivo assessment of artery smooth muscle [Ca2+]i and MLCK activation in FRET-based biosensor mice.American journal of physiology. Heart and circulatory physiology · 2010Article
- Activation by Ca2+/calmodulin of an exogenous myosin light chain kinase in mouse arteries.The Journal of physiology · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
FRET (Forster resonance energy transfer)-based biosensor molecules are powerful tools to reveal specific molecular interactions in cells. Typically however, they are used in cultured cells that (inevitably) express different genes than their counterparts in intact organisms. In such cells it may be impossible to administer physiological stimuli and measure physiological outputs. Here, through the use of transgenic mice that express a FRET-based myosin light chain kinase (MLCK) biosensor molecule, we report a technique for dynamically observing activation and regulation of MLCK within the smooth muscle cells of intact, functioning small arteries, together with measurement of arterial force production and intracellular [Ca(2+)].
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.