ArticleMolecular and biochemical parasitology2008
Fasciola hepatica expresses multiple alpha- and beta-tubulin isotypes.
Article in Molecular and biochemical parasitology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 53 citations in OpenAlex.
- A practical miracidial motility assay for assessing Fasciola hepatica sensitivity to compounds in vitro.International journal for parasitology. Drugs and drug resistance · 2026Article
- Protein Modelling and Molecular Docking Analysis of Fasciola hepatica β-Tubulin's Interaction Sites, with Triclabendazole, Triclabendazole Sulphoxide and Triclabendazole Sulphone.Acta parasitologica · 2023Article
- The 22nd Chromatography Component of theAnimals : an open access journal from MDPI · 2023Article
- A major locus confers triclabendazole resistance in Fasciola hepatica and shows dominant inheritance.PLoS pathogens · 2023Article
- Drug resistance in liver flukes.International journal for parasitology. Drugs and drug resistance · 2020Review
- Preliminary Evidence for the Occurrence of β-tubulin Isotype 3 Polymorphisms inHelminthologia · 2019Article
- Proteomic analysis of Fasciola gigantica excretory and secretory products (FgESPs) interacting with buffalo serum of different infection periods by shotgun LC-MS/MS.Parasitology research · 2019Article
- Pleiotropic alterations in gene expression in Latin American Fasciola hepatica isolates with different susceptibility to drugs.Parasites & vectors · 2018Article
- The Fasciola hepatica genome: gene duplication and polymorphism reveals adaptation to the host environment and the capacity for rapid evolution.Genome biology · 2015Article
- UDP-galactose 4'-epimerase from the liver fluke, Fasciola hepatica: biochemical characterization of the enzyme and identification of inhibitors.Parasitology · 2015Article
- Recent advances in candidate-gene and whole-genome approaches to the discovery of anthelmintic resistance markers and the description of drug/receptor interactions.International journal for parasitology. Drugs and drug resistance · 2014Review
- Liver fluke β-tubulin isotype 2 binds albendazole and is thus a probable target of this drug.Parasitology research · 2010Article
- Identification of two beta-tubulin isotypes of Clonorchis sinensis.Parasitology research · 2009Article
- Albendazole enantiomeric metabolism and binding to cytosolic proteins in the liver fluke Fasciola hepatica.Veterinary research communications · 2009Article
- Morphological response of triclabendazole-susceptible and triclabendazole-resistant isolates of Fasciola hepatica to treatment in vitro with nitroxynil (Trodax).Parasitology research · 2009Article
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Authors and funding
13 authors at 5 institutions in 1 country.
Funding
Abstract
We have identified five alpha-tubulin and six beta-tubulin isotypes that are expressed in adult Fasciola hepatica. Amino acid sequence identities ranged between 72 and 95% for fluke alpha-tubulin and between 65 and 97% for beta-tubulin isotypes. Nucleotide sequence identity ranged between 68-77% and 62-80%, respectively, for their coding sequences. Phylogenetic analysis indicated that two of the alpha-tubulins and two of the beta-tubulins were distinctly divergent from the other trematode and nematode tubulin sequences described in this study, whereas the other isotypes segregated within the trematode clades. With regard to the proposed benzimidazole binding site on beta-tubulin, three of the fluke isotypes had tyrosine at position 200 of beta-tubulin, two had phenylalanine and one had leucine. All had phenylalanine at position 167 and glutamic acid at position 198. When isotype RT-PCR fragment sequences were compared between six individual flukes from the susceptible Cullompton isolate and from seven individual flukes from the two resistant isolates, Sligo and Oberon, these residues were conserved.
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