ArticleInternational journal of legal medicine2008
'Mitominis': multiplex PCR analysis of reduced size amplicons for compound sequence analysis of the entire mtDNA control region in highly degraded samples.
Article in International journal of legal medicine, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Review
- A Rapid, Reliable and Reproducible Protocol for DNA Degradation in Genetic Applications.Cells · 2025Article
- Effects of solvent-based adhesive removal on the subsequent dual analysis of fingerprint and DNA.International journal of legal medicine · 2023Article
- DNA and protein analyses of hair in forensic genetics.International journal of legal medicine · 2023Review
- Evidence for multi-copy Mega-NUMTs in the human genome.Nucleic acids research · 2021Article
- Article
- Developmental Validation of a MPS Workflow with a PCR-Based Short Amplicon Whole Mitochondrial Genome Panel.Genes · 2020Article
- Massive parallel sequencing in forensics: advantages, issues, technicalities, and prospects.International journal of legal medicine · 2020Review
- Mitigating the effects of reference sequence bias in single-multiplex massively parallel sequencing of the mitochondrial DNA control region.Forensic science international. Genetics · 2019Article
- Singleplex quantitative real-time PCR for the assessment of human mitochondrial DNA quantity and quality.Forensic science, medicine, and pathology · 2018Article
- Article
- Results of a collaborative study on DNA identification of aged bone samples.Croatian medical journal · 2017Article
- DNA and RNA profiling of excavated human remains with varying postmortem intervals.International journal of legal medicine · 2016Article
- Simultaneous analysis of nuclear and mitochondrial DNA, mRNA and miRNA from backspatter from inside parts of firearms generated by shots at "triple contrast" doped ballistic models.Forensic science, medicine, and pathology · 2015Article
- Forensic identification of severely degraded Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss) tissues.Investigative genetics · 2010Article
- qPCR and mtDNA SNP analysis of experimentally degraded hair samples and its application in forensic casework.International journal of legal medicine · 2010Article
- Sequencing strategy for the whole mitochondrial genome resulting in high quality sequences.BMC genomics · 2009Article
- Mystery solved: the identification of the two missing Romanov children using DNA analysis.PloS one · 2009Article
- A rapid mtDNA assay of 22 SNPs in one multiplex reaction increases the power of forensic testing in European Caucasians.International journal of legal medicine · 2008Article
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The traditional protocol for forensic mitochondrial DNA (mtDNA) analyses involves the amplification and sequencing of the two hypervariable segments HVS-I and HVS-II of the mtDNA control region. The primers usually span fragment sizes of 300-400 bp each region, which may result in weak or failed amplification in highly degraded samples. Here we introduce an improved and more stable approach using shortened amplicons in the fragment range between 144 and 237 bp. Ten such amplicons were required to produce overlapping fragments that cover the entire human mtDNA control region. These were co-amplified in two multiplex polymerase chain reactions and sequenced with the individual amplification primers. The primers were carefully selected to minimize binding on homoplasic and haplogroup-specific sites that would otherwise result in loss of amplification due to mis-priming. The multiplexes have successfully been applied to ancient and forensic samples such as bones and teeth that showed a high degree of degradation.
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