ArticleEMBO reports2008
Substrate-induced DNA strand misalignment during catalytic cycling by DNA polymerase lambda.
Article in EMBO reports, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed.
- Fidelity, specialization, and evolution of Paramecium PolX DNA polymerases involved in programmed double-strand break DNA repair.Nucleic acids research · 2025Article
- Biology and Regulation of Staphylococcal Biofilm.International journal of molecular sciences · 2023Review
- Watching right and wrong nucleotide insertion captures hidden polymerase fidelity checkpoints.Nature communications · 2022Article
- Watching a double strand break repair polymerase insert a pro-mutagenic oxidized nucleotide.Nature communications · 2021Article
- Fidelity of a Bacterial DNA Polymerase in Microgravity, a Model for Human Health in Space.Frontiers in cell and developmental biology · 2021Article
- Structure and function relationships in mammalian DNA polymerases.Cellular and molecular life sciences : CMLS · 2020Review
- Structural basis for the binding and incorporation of nucleotide analogs with L-stereochemistry by human DNA polymerase λ.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Structures of the Leishmania infantum polymerase beta.DNA repair · 2014Article
- Computational simulation strategies for analysis of multisubunit RNA polymerases.Chemical reviews · 2013Review
- Mutation rates, spectra, and genome-wide distribution of spontaneous mutations in mismatch repair deficient yeast.G3 (Bethesda, Md.) · 2013Article
- Translesion DNA synthesis and mutagenesis in eukaryotes.Cold Spring Harbor perspectives in biology · 2013Review
- "Gate-keeper" residues and active-site rearrangements in DNA polymerase μ help discriminate non-cognate nucleotides.PLoS computational biology · 2013Article
- Structures of dNTP intermediate states during DNA polymerase active site assembly.Structure (London, England : 1993) · 2012Article
- In vitro gap-directed translesion DNA synthesis of an abasic site involving human DNA polymerases epsilon, lambda, and beta.The Journal of biological chemistry · 2011Article
- The in vitro fidelity of yeast DNA polymerase δ and polymerase ε holoenzymes during dinucleotide microsatellite DNA synthesis.DNA repair · 2011Article
- Biomolecularmodeling and simulation: a field coming of age.Quarterly reviews of biophysics · 2011Review
- Replication infidelity via a mismatch with Watson-Crick geometry.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Efficiency and fidelity of human DNA polymerases λ and β during gap-filling DNA synthesis.DNA repair · 2011Article
- Identification of critical residues for the tight binding of both correct and incorrect nucleotides to human DNA polymerase λ.Journal of molecular biology · 2010Article
- DNA pol λ's extraordinary ability to stabilize misaligned DNA.Journal of the American Chemical Society · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
The simple deletion of nucleotides is common in many organisms. It can be advantageous when it activates genes beneficial to microbial survival in adverse environments, and deleterious when it mutates genes relevant to survival, cancer or degenerative diseases. The classical idea is that simple deletions arise by strand slippage. A prime opportunity for slippage occurs during DNA synthesis, but it remains unclear how slippage is controlled during a polymerization cycle. Here, we report crystal structures and molecular dynamics simulations of mutant derivatives of DNA polymerase lambda bound to a primer-template during strand slippage. Relative to the primer strand, the template strand is in multiple conformations, indicating intermediates on the pathway to deletion mutagenesis. Consistent with these intermediates, the mutant polymerases generate single-base deletions at high rates. The results indicate that dNTP-induced template strand repositioning during conformational rearrangements in the catalytic cycle is crucial to controlling the rate of strand slippage.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.