ArticleRNA (New York, N.Y.)2008
Maturation of the 5S rRNA 5' end is catalyzed in vitro by the endonuclease tRNase Z in the archaeon H. volcanii.
Article in RNA (New York, N.Y.), 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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24 citing papers in PubMed, 40 citations in OpenAlex.
- Archaeal RNA processing and regulation: expanding the functional landscape.Microbiology and molecular biology reviews : MMBR · 2025Review
- RNase W, a conserved ribonuclease family with a novel active site.Nucleic acids research · 2024Article
- Endonucleolytic processing plays a critical role in the maturation of ribosomal RNA inRNA biology · 2023Article
- Comprehensive analysis of the pre-ribosomal RNA maturation pathway in a methanoarchaeon exposes the conserved circularization and linearization mode in archaea.RNA biology · 2020Article
- Splicing Endonuclease Is an Important Player in rRNA and tRNA Maturation in Archaea.Frontiers in microbiology · 2020Article
- An archaeal RNA binding protein, FAU-1, is a novel ribonuclease related to rRNA stability in Pyrococcus and Thermococcus.Scientific reports · 2017Article
- High-throughput sequencing reveals circular substrates for an archaeal RNA ligase.RNA biology · 2017Article
- An active immune defense with a minimal CRISPR (clustered regularly interspaced short palindromic repeats) RNA and without the Cas6 protein.The Journal of biological chemistry · 2015Article
- Biofilms formed by the archaeon Haloferax volcanii exhibit cellular differentiation and social motility, and facilitate horizontal gene transfer.BMC biology · 2014Article
- Extracellular DNA metabolism in Haloferax volcanii.Frontiers in microbiology · 2014Article
- Generation and phenotyping of a collection of sRNA gene deletion mutants of the haloarchaeon Haloferax volcanii.PloS one · 2014Article
- Essential requirements for the detection and degradation of invaders by the Haloferax volcanii CRISPR/Cas system I-B.RNA biology · 2013Article
- Dihydroxyacetone metabolism in Haloferax volcanii.Frontiers in microbiology · 2013Article
- An archaeal immune system can detect multiple protospacer adjacent motifs (PAMs) to target invader DNA.The Journal of biological chemistry · 2012Article
- High throughput sequencing reveals a plethora of small RNAs including tRNA derived fragments in Haloferax volcanii.RNA biology · 2012Article
- Assigning a function to a conserved archaeal metallo-β-lactamase from Haloferax volcanii.Extremophiles : life under extreme conditions · 2012Article
- Identification of essential and non-essential single-stranded DNA-binding proteins in a model archaeal organism.Nucleic acids research · 2012Article
- The archaeal Lsm protein binds to small RNAs.The Journal of biological chemistry · 2010Article
- tRNA biology charges to the front.Genes & development · 2010Review
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ribosomal RNA molecules are synthesized as precursors that have to undergo several processing steps to generate the functional rRNA. The 5S rRNA in the archaeon Haloferax volcanii is transcribed as part of a multicistronic transcript containing both large rRNAs and one or two tRNAs. Release of the 5S rRNA from the precursor requires two endonucleolytic cleavages by enzymes as yet not identified. Here we report the first identification of an archaeal 5S rRNA processing endonuclease. The enzyme tRNase Z, which was initially identified as tRNA processing enzyme, generates not only tRNA 3' ends but also mature 5S rRNA 5' ends in vitro. Interestingly, the sequence upstream of the 5S rRNA can be folded into a mini-tRNA, which might explain the processing of this RNA by tRNase Z. The endonuclease is active only at low salt concentrations in vitro, which is in contrast to the 2-4 M KCl concentration present inside the cell in vivo. Electron microscopy studies show that there are no compartments inside the Haloferax cell that could provide lower salt environments. Processing of the 5S rRNA 5' end is not restricted to the haloarchaeal tRNase Z since tRNase Z enzymes from a thermophilic archaeon, a lower and a higher eukaryote, are as well able to cleave the tRNA-like structure 5' of the 5S rRNA. Knock out of the tRNase Z gene in Haloferax volcanii is lethal, showing that the protein is essential for the cell.
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