ArticleStem cells (Dayton, Ohio)2008
Copy number variant analysis of human embryonic stem cells.
Article in Stem cells (Dayton, Ohio), 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 59 citations in OpenAlex.
- Altered genome induced immune response of iPSCs.Frontiers in immunology · 2026Article
- A reference human induced pluripotent stem cell line for large-scale collaborative studies.Cell stem cell · 2022Article
- New hPSC SOX9 and INS Reporter Cell Lines Facilitate the Observation and Optimization of Differentiation into Insulin-Producing Cells.Stem cell reviews and reports · 2021Article
- Miniaturized 3D bone marrow tissue model to assess response to Thrombopoietin-receptor agonists in patients.eLife · 2021Article
- Genomic integrity of human induced pluripotent stem cells across nine studies in the NHLBI NextGen program.Stem cell research · 2020Article
- Gain of 20q11.21 in Human Pluripotent Stem Cells Impairs TGF-β-Dependent Neuroectodermal Commitment.Stem cell reports · 2019Article
- Verification of Long-Term Genetic Stability of hMSCs during Subculture after Internalization of Sunflower-Type Nanoparticles (SF-NPs).Theranostics · 2018Article
- High-Throughput and Cost-Effective Characterization of Induced Pluripotent Stem Cells.Stem cell reports · 2017Article
- Influence of ATM-Mediated DNA Damage Response on Genomic Variation in Human Induced Pluripotent Stem Cells.Stem cells and development · 2016Article
- Genomic instability of human embryonic stem cell lines using different passaging culture methods.Molecular cytogenetics · 2015Article
- Somatic copy number mosaicism in human skin revealed by induced pluripotent stem cells.Nature · 2012Article
- Genetic and epigenetic stability of human pluripotent stem cells.Nature reviews. Genetics · 2012Review
- Investigating the role of X chromosome breakpoints in premature ovarian failure.Molecular cytogenetics · 2012Article
- Human embryonic stem cell responses to ionizing radiation exposures: current state of knowledge and future challenges.Stem cells international · 2012Article
- High-Resolution Genomic Profiling of Chromosomal Abnormalities in Human Stem Cells Using the 135 K StemArray.Stem cells international · 2012Article
- PTK7 marks the first human developmental EMT in vitro.PloS one · 2012Article
- Screening ethnically diverse human embryonic stem cells identifies a chromosome 20 minimal amplicon conferring growth advantage.Nature biotechnology · 2011Article
- Sustained embryoid body formation and culture in a non-laborious three dimensional culture system for human embryonic stem cells.Cytotechnology · 2011Article
- Dynamic changes in the copy number of pluripotency and cell proliferation genes in human ESCs and iPSCs during reprogramming and time in culture.Cell stem cell · 2011Article
- Safety paradigm: genetic evaluation of therapeutic grade human embryonic stem cells.Journal of the Royal Society, Interface · 2010Review
Corrections and comments
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Authors and funding
17 authors at 5 institutions in 1 country.
Funding
Abstract
Differences between individual DNA sequences provide the basis for human genetic variability. Forms of genetic variation include single-nucleotide polymorphisms, insertions/duplications, deletions, and inversions/translocations. The genome of human embryonic stem cells (hESCs) has been characterized mainly by karyotyping and comparative genomic hybridization (CGH), techniques whose relatively low resolution at 2-10 megabases (Mb) cannot accurately determine most copy number variability, which is estimated to involve 10%-20% of the genome. In this brief technical study, we examined HSF1 and HSF6 hESCs using array-comparative genomic hybridization (aCGH) to determine copy number variants (CNVs) as a higher-resolution method for characterizing hESCs. Our approach used five samples for each hESC line and showed four consistent CNVs for HSF1 and five consistent CNVs for HSF6. These consistent CNVs included amplifications and deletions that ranged in size from 20 kilobases to 1.48 megabases, involved seven different chromosomes, were both shared and unique between hESCs, and were maintained during neuronal stem/progenitor cell differentiation or drug selection. Thirty HSF1 and 40 HSF6 less consistently scored but still highly significant candidate CNVs were also identified. Overall, aCGH provides a promising approach for uniquely identifying hESCs and their derivatives and highlights a potential genomic source for distinct differentiation and functional potentials that lower-resolution karyotype and CGH techniques could miss. Disclosure of potential conflicts of interest is found at the end of this article.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.