ArticlePlant cell reports2008
In planta transformation of Notocactus scopa cv. Soonjung by Agrobacterium tumefaciens.
Article in Plant cell reports, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Construction and Segmental Reconstitution of Full-Length Infectious Clones of Milk Vetch Dwarf Virus.Viruses · 2025Article
- A comprehensive review of in planta stable transformation strategies.Plant methods · 2024Review
- Insights into the Differential Composition of Stem-Loop Structures of Nanoviruses and Their Impacts.Microbiology spectrum · 2023Article
- Article
- Identification of a Novel Geminivirus inViruses · 2021Article
- Effects of light intensity and plant growth regulators on callus proliferation and shoot regeneration in the ornamental succulent Haworthia.Botanical studies · 2019Article
- Agrobacterium-mediated transient transformation of Bienertia sinuspersici to assay recombinant protein distribution between dimorphic chloroplasts.Plant cell reports · 2019Article
- Agrobacterium-mediated in planta genetic transformation of sugarcane setts.Plant cell reports · 2015Article
- An efficient in planta transformation of Jatropha curcas (L.) and multiplication of transformed plants through in vivo grafting.Protoplasma · 2014Article
- Agrobacterium tumefaciens-mediated in planta seed transformation strategy in sugarcane.Plant cell reports · 2013Article
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7 authors.
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Abstract
Notocactus scopa cv. Soonjung was subjected to in planta Agrobacterium tumefaciens-mediated transformation with vacuum infiltration, pin-pricking, and a combination of the two methods. The pin-pricking combined with vacuum infiltration (20-30 cmHg for 15 min) resulted in a transformation efficiency of 67-100%, and the expression of the uidA and nptII genes was detected in transformed cactus. The established in planta transformation technique generated a transgenic cactus with higher transformation efficiency, shortened selection process, and stable gene expression via asexual reproduction. All of the results showed that the in planta transformation method utilized in the current study provided an efficient and time-saving procedure for the delivery of genes into the cactus genome, and that this technique can be applied to other asexually reproducing succulent plant species.
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