ArticleAmerican journal of respiratory cell and molecular biology2008
Functional live cell imaging of the pulmonary neuroepithelial body microenvironment.
Article in American journal of respiratory cell and molecular biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 61 citations in OpenAlex.
- Are pulmonary neuroepithelial bodies sensors for acute airway hypoxia implicated in the central regulation of breathing?PloS one · 2026Article
- Advances in pulmonary neuroendocrine cells and their cellular interactions in asthma pathogenesis.Frontiers in pharmacology · 2025Review
- Investigating pulmonary neuroendocrine cells in human respiratory diseases with airway models.Disease models & mechanisms · 2024Review
- KBiomedicines · 2023Review
- Pulmonary Sensory Receptors.Advances in anatomy, embryology, and cell biology · 2021Article
- Functional Exploration of the Pulmonary NEB ME.Advances in anatomy, embryology, and cell biology · 2021Article
- The Pulmonary NEB ME Is a Complex Intraepithelial Unit.Advances in anatomy, embryology, and cell biology · 2021Article
- Studying the Pulmonary NEB ME: A Multidisciplinary Approach.Advances in anatomy, embryology, and cell biology · 2021Article
- Pulmonary neuroendocrine cells: physiology, tissue homeostasis and disease.Disease models & mechanisms · 2020Review
- Article
- Mitochondria: at the crossroads of regulating lung epithelial cell function in chronic obstructive pulmonary disease.American journal of physiology. Lung cellular and molecular physiology · 2020Review
- The Basic Study of the Mechanism of Propofol-Related Infusion Syndrome Using a Murine Skeletal Muscle Injury Model.Anesthesiology and pain medicine · 2019Article
- Ontogeny and Biology of Human Small Airway Epithelial Club Cells.American journal of respiratory and critical care medicine · 2018Article
- Selective activation and proliferation of a quiescent stem cell population in the neuroepithelial body microenvironment.Respiratory research · 2018Article
- Myostatin knockout induces apoptosis in human cervical cancer cells via elevated reactive oxygen species generation.Redox biology · 2018Article
- A novel inhibitor of inducible NOS dimerization protects against cytokine-induced rat beta cell dysfunction.British journal of pharmacology · 2018Article
- Selective gene expression analysis of the neuroepithelial body microenvironment in postnatal lungs with special interest for potential stem cell characteristics.Respiratory research · 2017Article
- Article
- Mechanisms underlying apoptosis-inducing effects of Kaempferol in HT-29 human colon cancer cells.International journal of molecular sciences · 2014Article
- Article
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8 authors at 3 institutions in 3 countries.
Funding
Abstract
Pulmonary neuroepithelial bodies (NEBs) are densely innervated groups of neuroendocrine cells invariably accompanied by Clara-like cells. Together with NEBs, Clara-like cells form the so-called "NEB microenvironment," which recently has been assigned a potential pulmonary stem cell niche. Conclusive data on the nature of physiological stimuli for NEBs are lacking. This study aimed at developing an ex vivo mouse lung vibratome slice model for confocal live cell imaging of physiological reactions in identified NEBs and surrounding epithelial cells. Immunohistochemistry of fixed slices demonstrated that NEBs are almost completely shielded from the airway lumen by tight junction-linked Clara-like cells. Besides the unambiguous identification of NEBs, the fluorescent dye 4-Di-2-ASP allowed microscopic identification of ciliated cells, Clara cells, and Clara-like cells in live lung slices. Using the mitochondrial uncoupler FCCP and a mitochondrial membrane potential indicator, JC-1, increases in 4-Di-2-ASP fluorescence in NEB cells and ciliated cells were shown to represent alterations in mitochondrial membrane potential. Changes in the intracellular free calcium concentration ([Ca2+](i)) in NEBs and surrounding airway epithelial cells were simultaneously monitored using the calcium indicator Fluo-4. Application (5 s) of 50 mM extracellular potassium ([K+](o)) evoked a fast and reproducible [Ca2+](i) increase in NEB cells, while Clara-like cells displayed a delayed (+/- 4 s) [Ca2+](i) increase, suggestive of an indirect, NEB-mediated activation. The presented approach opens interesting new perspectives for unraveling the functional significance of pulmonary NEBs in control lungs and disease models, and for the first time allows direct visualization of local interactions within the NEB microenvironment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.