ArticleRNA (New York, N.Y.)2008
Hairpin RNAs derived from RNA polymerase II and polymerase III promoter-directed transgenes are processed differently in plants.
Article in RNA (New York, N.Y.), 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 3 citations in OpenAlex.
- Enhancement of the CRISPR/Cas9-Based Genome Editing System in Lettuce (Plants (Basel, Switzerland) · 2023Article
- Molecular mechanisms underlying host-induced gene silencing.The Plant cell · 2022Review
- Nucleotide mismatches prevent intrinsic self-silencing of hpRNA transgenes to enhance RNAi stability in plants.Nature communications · 2022Article
- Improvement of host-induced gene silencing efficiency via polycistronic-tRNA-amiR expression for multiple target genes and characterization of RNAi mechanism in Mythimna separata.Plant biotechnology journal · 2021Article
- Rolling Circle Amplification (RCA)-Mediated Genome-Wide ihpRNAi Mutant Library Construction inInternational journal of molecular sciences · 2020Article
- Progresses, Challenges, and Prospects of Genome Editing in Soybean (Frontiers in plant science · 2020Review
- Full-Length Hairpin RNA Accumulates at High Levels in Yeast but Not in Bacteria and Plants.Genes · 2019Article
- Optimization of CRISPR/Cas9 genome editing in cotton by improved sgRNA expression.Plant methods · 2018Article
- Article
- Expression Analysis of Hairpin RNA CarryingBioMed research international · 2017Article
- DNA-Demethylase Regulated Genes Show Methylation-Independent Spatiotemporal Expression Patterns.Frontiers in plant science · 2017Article
- RNA Silencing in Plants: Mechanisms, Technologies and Applications in Horticultural Crops.Current genomics · 2016Article
- Parameters affecting frequency of CRISPR/Cas9 mediated targeted mutagenesis in rice.Plant cell reports · 2015Article
- Comparison of CRISPR/Cas9 expression constructs for efficient targeted mutagenesis in rice.Plant molecular biology · 2015Article
- CRISPRing into the woods.GM crops & food · 2015Article
- Nicotiana small RNA sequences support a host genome origin of cucumber mosaic virus satellite RNA.PLoS genetics · 2015Article
- Satellite RNAs interfere with the function of viral RNA silencing suppressors.Frontiers in plant science · 2015Article
- DNA demethylases target promoter transposable elements to positively regulate stress responsive genes in Arabidopsis.Genome biology · 2014Article
- Article
- Comparative profiling of miRNA expression in developing seeds of high linoleic and high oleic safflower (Carthamus tinctorius L.) plants.Frontiers in plant science · 2013Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA polymerase III (Pol III) as well as Pol II (35S) promoters are able to drive hairpin RNA (hpRNA) expression and induce target gene silencing in plants. siRNAs of 21 nt are the predominant species in a 35S Pol II line, whereas 24- and/or 22-nucleotide (nt) siRNAs are produced by a Pol III line. The 35S line accumulated the loop of the hpRNA, in contrast to full-length hpRNA in the Pol III line. These suggest that Pol II and Pol III-transcribed hpRNAs are processed by different pathways. One Pol III transgene produced only 24-nt siRNAs but silenced the target gene efficiently, indicating that the 24-nt siRNAs can direct mRNA degradation; specific cleavage was confirmed by 5' rapid amplification of cDNA ends (RACE). Both Pol II- and Pol III-directed hpRNA transgenes induced cytosine methylation in the target DNA. The extent of methylation is not correlated with the level of 21-nt siRNAs, suggesting that they are not effective inducers of DNA methylation. The promoter of a U6 transgene was significantly methylated, whereas the promoter of the endogenous U6 gene was almost free of cytosine methylation, suggesting that endogenous sequences are more resistant to de novo DNA methylation than are transgene constructs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.