ArticleJournal of clinical microbiology2008
Development and validation of a one-step real-time PCR assay for simultaneous detection of subtype H5, H7, and H9 avian influenza viruses.
Article in Journal of clinical microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 80 papers.
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Who cites it
80 citing papers in PubMed.
- The Role of Wildlife and Climate Change in the Emergence of One Health-Related Viral Diseases in Australia.Viruses · 2026Review
- Molecular characterization and pathogenicity of H9N2 avian influenza viruses in poultry in Shandong Province, China, 2021-2023.Poultry science · 2026Article
- Development of multiplex real-time RT‒PCR assays for the simultaneous detection and lineage differentiation of H9 avian influenza viruses.Poultry science · 2026Article
- Genomic characterization of H6N1 avian influenza viruses from wild birds and domestic ducks in the republic of Korea and Mongolia (2022-2024).Archives of virology · 2026Article
- A Cross-Sectional Virological and Sero-Epidemiological Study of Exposures to Avian Influenza A(H5N1) and A(H9N2) Viruses in Live Bird Market Workers in Dhaka, Bangladesh.Influenza and other respiratory viruses · 2025Article
- Article
- Development and Laboratory Validation of Rapid, Bird-Side Molecular Diagnostic Assays for Avian Influenza Virus Including Panzootic H5Nx.Microorganisms · 2025Article
- Prevalence and risk factors of Influenza Avian Virus in backyard pigeons, ducks, and chickens in Toba Tek Singh District, Pakistan.PloS one · 2025Article
- Article
- Diversity of the H9N2 Avian Influenza Virus in Shandong Province, China.Transboundary and emerging diseases · 2025Article
- First detection of D181 genotype of infectious bronchitis in poultry flocks of Morocco.Virology journal · 2024Article
- Genotype classification and pathogenicity of infectious bursal disease virus circulating in vaccinated broiler chicken farms.Veterinary research communications · 2024Article
- Genetic insights of H9N2 avian influenza viruses circulating in Mali and phylogeographic patterns in Northern and Western Africa.Virus evolution · 2024Article
- First Report of Low Pathogenic Avian Influenza Subtype H9N2 in African Houbara Bustards (Viruses · 2023Article
- Low Pathogenic Avian Influenza H9N2 Viruses in Morocco: Antigenic and Molecular Evolution from 2021 to 2023.Viruses · 2023Article
- Development of a Novel Korean H9-Specific rRT-PCR Assay and Its Application for Avian Influenza Virus Surveillance in Korea.Journal of microbiology (Seoul, Korea) · 2023Article
- Prevalence of major infectious diseases in backyard chickens from rural markets in Morocco.Veterinary world · 2023Article
- The Evolution of Highly Pathogenic Avian Influenza A (H5) in Poultry in Nigeria, 2021-2022.Viruses · 2023Article
- Risk Factors Associated with Avian Influenza Subtype H9 Outbreaks in Poultry Farms of Central Lowland Nepal.Infectious disease reports · 2022Article
- Redesign and Validation of a Real-Time RT-PCR to Improve Surveillance for Avian Influenza Viruses of the H9 Subtype.Viruses · 2022Article
20 more citing papers are in PubMed but not listed here.
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10 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Among the different hemagglutinin (HA) subtypes of avian influenza (AI) viruses, H5, H7, and H9 are of major interest because of the serious consequences for the poultry industry and the increasing frequency of direct transmission of these viruses to humans. The availability of new tools to rapidly detect and subtype the influenza viruses can enable the immediate application of measures to prevent the widespread transmission of the infection. In this study, a novel one-step real-time reverse transcription-PCR (RRT-PCR) was developed to detect simultaneously the H5, H7, and H9 subtypes of AI viruses from clinical samples of avian origin. The sensitivity of the RRT-PCR assay was determined by using in vitro-transcribed RNA and 10-fold serial dilutions of titrated AI viruses. High sensitivity levels were obtained, with limits of detection ranging from 10(1) to 10(3) RNA copies and from 10(1) 50% egg infectious dose (EID(50))/100 microl to 10(2.74) EID(50)/100 microl with titrated viruses. Excellent results were achieved in the intra- and interassay variability tests. The comparison of the results with those obtained from the analysis of 725 avian samples by means of the reference method (virus isolation [VI]) showed a high level of agreement. To date, this is the first real-time PCR protocol available for the simultaneous detection of AI viruses belonging to subtypes H5, H7, and H9, and the results obtained indicate that this method is suitable as a routine laboratory test for the rapid detection and differentiation of the three most-important AI virus subtypes in samples of avian origin.
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