ArticleJournal of clinical microbiology2008
Comparison of serological and real-time PCR assays to diagnose Bordetella pertussis infection in 2007.
Article in Journal of clinical microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- Guidelines for the management of adult lower respiratory tract infections--full version.Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases · 2011Guideline
- What to do and what not to do in serological diagnosis of pertussis: recommendations from EU reference laboratories.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2011Guideline
- Etiology of community-acquired pneumonia and diagnostic yields of microbiological methods: a 3-year prospective study in Norway.BMC infectious diseases · 2015Trial
- Direct Detection ofSensors (Basel, Switzerland) · 2025Article
- Development and implementation of standardized method for detecting immunogenicity of acellular pertussis vaccines in Korea.Clinical and experimental vaccine research · 2019Article
- Article
- Molecular detection ofIranian journal of microbiology · 2017Article
- Epidemiology of pertussis in Casablanca (Morocco): contribution of conventional and molecular diagnosis tools.BMC infectious diseases · 2017Article
- Protecting Newborns Against Pertussis: Treatment and Prevention Strategies.Paediatric drugs · 2015Review
- Laboratory Diagnosis of Pertussis.Clinical microbiology reviews · 2015Review
- Incidence of pertussis in patients of general practitioners in Poland.Epidemiology and infection · 2014Article
- Insertion sequences shared by Bordetella species and implications for the biological diagnosis of pertussis syndrome.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2013Article
- Two-component cluster analysis of a large serodiagnostic database for specificity of increases of IgG antibodies against pertussis toxin in paired serum samples and of absolute values in single serum samples.Clinical and vaccine immunology : CVI · 2012Article
- Clinical definitions of pertussis: Summary of a Global Pertussis Initiative roundtable meeting, February 2011.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2012Article
- Dual infection with Bordetella pertussis and Mycoplasma pneumoniae in three infants: case reports.Infection · 2012Article
- Prospective evaluation of an Australian pertussis toxin IgG and IgA enzyme immunoassay.Clinical and vaccine immunology : CVI · 2012Article
- Detection of Bordetella pertussis in Infants Suspected to have Whooping Cough.The open respiratory medicine journal · 2012Article
- Comparative study of different sources of pertussis toxin (PT) as coating antigens in IgG anti-PT enzyme-linked immunosorbent assays.Clinical and vaccine immunology : CVI · 2012Article
- Significant finding of Bordetella holmesii DNA in nasopharyngeal samples from French patients with suspected pertussis.Journal of clinical microbiology · 2011Article
- Evaluation of four commercial real-time PCR assays for detection of Bordetella spp. in nasopharyngeal aspirates.Journal of clinical microbiology · 2011Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacterial culture for diagnosing pertussis infection has high specificity but poor sensitivity and is slow. Highly sensitive real-time PCR assays and single-serum pertussis serology have been developed to overcome these limitations, but there are few data available on the relative sensitivities and specificities of such assays for pertussis diagnosis. Using data on 195 participants (>or=7 years old) from an epidemiological study, we assessed the sensitivity, specificity, and performance (Youden index) for pertussis diagnosis of the pertussis toxin enzyme-linked immunosorbent assay (using single and paired serology) and of real-time PCR assays (using the IS481 and ptxA-Pr targets). All available diagnostic information (clinical and laboratory) was pooled to serve as the gold standard. Single serology was the most efficient diagnostic test (Youden index, 0.57 to 0.58), with relatively high sensitivity (>64%) and high specificity (>90%), independent of the cutoff level. IS481 PCR performance was superior to that of ptxA-Pr PCR, and it was the second-most-efficient tool (Youden index, 0.30). Performing both ptxA-Pr and IS481 PCRs did not improve diagnostic performance. The greatest test efficiency (Youden index, 0.69 to 0.74) was achieved when single-serum serology was used in combination with IS481 or ptxA-Pr PCR or paired serology. Combining single serology with one PCR or paired serology increased the sensitivity with an associated limited decrease in specificity. The most specific tests for diagnosis of pertussis were single serology and ptxA-Pr PCR, and the most sensitive diagnostic tool was the combination of IS481 PCR with single serology.
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