ArticleBMC genomics2008
cDNA-AFLP analysis of plant and pathogen genes expressed in grapevine infected with Plasmopara viticola.
Article in BMC genomics, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
40 citing papers in PubMed, 122 citations in OpenAlex.
- The Carbonic Anhydrase βCA1 Functions in PopW-Mediated Plant Defense Responses in Tomato.International journal of molecular sciences · 2023Article
- Article
- Comparative transcriptomic profiling of susceptible and resistant cultivars of pigeonpea demonstrates early molecular responses during Fusarium udum infection.Scientific reports · 2021Article
- Investigation of long non-coding RNAs as regulatory players of grapevine response to powdery and downy mildew infection.BMC plant biology · 2021Article
- Review
- Plasmopara viticola infection affects mineral elements allocation and distribution in Vitis vinifera leaves.Scientific reports · 2020Article
- Semi-Targeted Metabolomics to Validate Biomarkers of Grape Downy Mildew Infection Under Field Conditions.Plants (Basel, Switzerland) · 2020Article
- Article
- Insight Into Function and Subcellular Localization ofFrontiers in microbiology · 2020Article
- Identification of Lipid Markers ofFrontiers in plant science · 2018Article
- Genome sequence of Plasmopara viticola and insight into the pathogenic mechanism.Scientific reports · 2017Article
- Article
- Antioxidant System Response and cDNA-SCoT Marker Profiling inInternational journal of genomics · 2017Article
- Grapevine Pathogenic Microorganisms: Understanding Infection Strategies and Host Response Scenarios.Frontiers in plant science · 2016Review
- Isolation and Characterization of Pepper Genes Interacting with the CMV-P1 Helicase Domain.PloS one · 2016Article
- Genetic diversity of stilbene metabolism in Vitis sylvestris.Journal of experimental botany · 2015Article
- The effect on the transcriptome of Anemone coronaria following infection with rust (Tranzschelia discolor).PloS one · 2015Article
- Article
- Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe oomycete Plasmopara viticola (Berk. and Curt.) Berl. and de Toni causes downy mildew in grapevine (Vitis vinifera L.). This pathogen is strictly biotrophic, thus completely dependent on living host cells for its survival. The molecular basis of compatibility and disease development in this system is poorly understood. We have carried out a large-scale cDNA-AFLP analysis to identify grapevine and P. viticola genes associated with the infection process.
resultsWe carried out cDNA-AFLP analysis on artificially infected leaves of the susceptible cultivar Riesling at the oil spot stage, on water-treated leaves and on a sample of pure sporangia as controls. Selective amplifications with 128 primer combinations allowed the visualization of about 7000 transcript-derived fragments (TDFs) in infected leaves, 1196 of which (17%) were differentially expressed. We sequenced 984 fragments, 804 of which were identified as grapevine transcripts after homology searching, while 96 were homologous to sequences in Phytophthora spp. databases and were attributed to P. viticola. There were 82 orphan TDFs. Many grapevine genes spanning almost all functional categories were downregulated during infection, especially genes involved in photosynthesis. Grapevine genes homologous to known resistance genes also tended to be repressed, as were several resistance gene analogs and carbonic anhydrase (recently implicated in pathogen resistance). In contrast, genes encoding cytoskeletal components, enzymes of the phenylpropanoid and beta-oxidation pathways, and pathogenesis related proteins were primarily upregulated during infection. The majority of P. viticola transcripts expressed in planta showed homology to genes of unknown function or to genomic Phytophthora sequences, but genes related to metabolism, energy production, transport and signal transduction were also identified.
conclusionThis study provides the first global catalogue of grapevine and P. viticola genes expressed during infection, together with their functional annotations. This will help to elucidate the molecular basis of the infection process and identify genes and chemicals that could help to inhibit the pathogen.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.