ArticleBMC genomics2008
Construction of a BAC library and mapping BAC clones to the linkage map of Barramundi, Lates calcarifer.
Article in BMC genomics, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 31 citations in OpenAlex.
- The FTO Gene Is Associated with Growth and Omega-3/-6 Ratio in Asian Seabass.Marine biotechnology (New York, N.Y.) · 2018Article
- BAC-pool sequencing and analysis confirms growth-associated QTLs in the Asian seabass genome.Scientific reports · 2016Article
- Microbial Consortium Associated with the Antarctic Marine Ciliate Euplotes focardii: An Investigation from Genomic Sequences.Microbial ecology · 2015Article
- Article
- Consolidation of the genetic and cytogenetic maps of turbot (Scophthalmus maximus) using FISH with BAC clones.Chromosoma · 2014Article
- Construction of a high-coverage bacterial artificial chromosome library and comprehensive genetic linkage map of yellowtail Seriola quinqueradiata.BMC research notes · 2014Article
- Analysis of two lysozyme genes and antimicrobial functions of their recombinant proteins in Asian seabass.PloS one · 2013Article
- Second-generation genetic linkage map of catfish and its integration with the BAC-based physical map.G3 (Bethesda, Md.) · 2012Article
- Article
- Analysis of the Asian seabass transcriptome based on expressed sequence tags.DNA research : an international journal for rapid publication of reports on genes and genomes · 2011Article
- BAC library resources for map-based cloning and physical map construction in barley (Hordeum vulgare L.).BMC genomics · 2011Article
- A high-resolution linkage map for comparative genome analysis and QTL fine mapping in Asian seabass, Lates calcarifer.BMC genomics · 2011Article
- Article
- Identification and analysis of immune-related transcriptome in Asian seabass Lates calcarifer.BMC genomics · 2010Article
- Escherichia coli MW005: lambda Red-mediated recombineering and copy-number induction of oriV-equipped constructs in a single host.BMC biotechnology · 2010Article
- Genome evolution in Reptilia: in silico chicken mapping of 12,000 BAC-end sequences from two reptiles and a basal bird.BMC genomics · 2009Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundBarramundi (Lates calcarifer) is an important farmed marine food fish species. Its first generation linkage map has been applied to map QTL for growth traits. To identify genes located in QTL responsible for specific traits, genomic large insert libraries are of crucial importance. We reported herein a bacterial artificial chromosome (BAC) library and the mapping of BAC clones to the linkage map.
resultsThis BAC library consisted of 49,152 clones with an average insert size of 98 kb, representing 6.9-fold haploid genome coverage. Screening the library with 24 microsatellites and 15 ESTs/genes demonstrated that the library had good genome coverage. In addition, 62 novel microsatellites each isolated from 62 BAC clones were mapped onto the first generation linkage map. A total of 86 BAC clones were anchored on the linkage map with at least one BAC clone on each linkage group.
conclusionWe have constructed the first BAC library for L. calcarifer and mapped 86 BAC clones to the first generation linkage map. This BAC library and the improved linkage map with 302 DNA markers not only supply an indispensable tool to the integration of physical and linkage maps, the fine mapping of QTL and map based cloning genes located in QTL of commercial importance, but also contribute to comparative genomic studies and eventually whole genome sequencing.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.