ArticleBMC evolutionary biology2008
Selection against tandem splice sites affecting structured protein regions.
Article in BMC evolutionary biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- An extended catalogue of tandem alternative splice sites in human tissue transcriptomes.PLoS computational biology · 2021Article
- Impact of acceptor splice site NAGTAG motif on exon recognition.Molecular biology reports · 2019Article
- Article
- Computational evidence of NAGNAG alternative splicing in human large intergenic noncoding RNA.BioMed research international · 2014Article
- Extensive regulation of NAGNAG alternative splicing: new tricks for the spliceosome?Genome biology · 2012Article
- Alternative splicing of RNA triplets is often regulated and accelerates proteome evolution.PLoS biology · 2012Article
- TassDB2 - A comprehensive database of subtle alternative splicing events.BMC bioinformatics · 2010Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAlternative selection of splice sites in tandem donors and acceptors is a major mode of alternative splicing. Here, we analyzed whether in-frame tandem sites leading to subtle mRNA insertions/deletions of 3, 6, or 9 nucleotides are under natural selection.
resultsWe found multiple lines of evidence that the human protein coding sequences are under selection against such in-frame tandem splice events, indicating that these events are often deleterious. The strength of selection is not homogeneous within the coding sequence as protein regions that fold into a fixed 3D structure (intrinsically ordered) are under stronger selection, especially against sites with a strong minor splice site. Investigating structures of functional protein domains, we found that tandem acceptors are preferentially located at the domain surface and outside structural elements such as helices and sheets. Using three-species comparisons, we estimate that more than half of all mutations that create NAGNAG acceptors in the coding region have been eliminated by selection.
conclusionWe estimate that ~2,400 introns are under selection against possessing a tandem site.
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Registered trials
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