ArticleNeurochemical research2008
Distinct subcellular distribution of delta-opioid receptor fused with various tags in PC12 cells.
Article in Neurochemical research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Knock-In Mouse Models to Investigate the Functions of Opioid ReceptorsFrontiers in cellular neuroscience · 2022Review
- PI3K class II α regulates δ-opioid receptor export from theMolecular biology of the cell · 2017Article
- A PTEN-Regulated Checkpoint Controls Surface Delivery of δ Opioid Receptors.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
- The delta opioid receptor tool box.Neuroscience · 2016Review
- Prospects for Creation of Cardioprotective and Antiarrhythmic Drugs Based on Opioid Receptor Agonists.Medicinal research reviews · 2016Review
- Molecular Pharmacology of δ-Opioid Receptors.Pharmacological reviews · 2016Review
- Tracking Drug-induced Changes in Receptor Post-internalization Trafficking by Colocalizational Analysis.Journal of visualized experiments : JoVE · 2015Article
- Opioid receptor trafficking and interaction in nociceptors.British journal of pharmacology · 2015Review
- Prolonged morphine treatment alters δ opioid receptor post-internalization trafficking.British journal of pharmacology · 2015Article
- Recent advances on the δ opioid receptor: from trafficking to function.British journal of pharmacology · 2015Review
- Molecular Perspectives for mu/delta Opioid Receptor Heteromers as Distinct, Functional Receptors.Cells · 2014Article
- Pharmacological traits of delta opioid receptors: pitfalls or opportunities?Psychopharmacology · 2013Review
- Chronic inflammatory injury results in increased coupling of delta opioid receptors to voltage-gated Ca2+ channels.Molecular pain · 2013Article
- Opioid receptor heteromers in analgesia.Expert reviews in molecular medicine · 2012Review
- Functional relevance of μ-δ opioid receptor heteromerization: a role in novel signaling and implications for the treatment of addiction disorders: from a symposium on new concepts in mu-opioid pharmacology.Drug and alcohol dependence · 2012Review
- Emergence of functional spinal delta opioid receptors after chronic ethanol exposure.Biological psychiatry · 2012Article
- Activation of spinal mu- and delta-opioid receptors potently inhibits substance P release induced by peripheral noxious stimuli.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- Transport of receptors, receptor signaling complexes and ion channels via neuropeptide-secretory vesicles.Cell research · 2011Article
- Prime time for G-protein-coupled receptor heteromers as therapeutic targets for CNS disorders: the dopamine D₁-D₃ receptor heteromer.CNS & neurological disorders drug targets · 2010Review
- Role of progerin-induced telomere dysfunction in HGPS premature cellular senescence.Journal of cell science · 2010Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In small dorsal root ganglion neurons, delta-opioid receptors (DORs) have been found to be mainly distributed in the cytoplasm and often associated with the membrane of large dense-core vesicles (LDCVs) that contain neuropeptides. To study the distribution of DORs under various physiological or pharmacological conditions, the receptors fused with different tags are constructed, transfected into cells or animals, and examined with microscopy. In this study, we show that DOR with different tags have distinct patterns of subcellular distribution in neuroendocrine cells, PC12 cells. Both immunostaining and vesicle fraction analysis showed that the native DORs expressed in PC12 cells were mainly associated with LDCVs. In transfected PC12 cells, DOR tagged with Myc or hemagglutinin exhibited LDCV localization. However, DOR fused with GFP at N- or C-terminus was found to be mainly localized on the cell surface, and mediated the function of DOR agonist. Therefore, the distribution of DOR fused with GFP differs from the native DORs. These results suggest that the subcellular distribution of the receptor could be better presented by the fused tag with smaller molecular size.
Indexed as
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18365312What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.