ArticleEuropean biophysics journal : EBJ2008
Probing elasticity and adhesion of live cells by atomic force microscopy indentation.
Article in European biophysics journal : EBJ, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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35 citing papers in PubMed, 130 citations in OpenAlex.
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- Cell wall-derived mechanical signals control cell growth and division during root development.Science advances · 2025Article
- Mechanics and disease of heart cells/cardiomyocytes explored through atomic force microscopy: present and future.Biophysical reviews · 2025Review
- Aberrant Chitinase 3-Like 1 Expression in Basal Cells Contributes to Systemic Sclerosis Fibrosis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Accurate Modelling of AFM Force-Indentation Curves with Blunted Indenters at Small Indentation Depths.Micromachines · 2024Article
- Rolling of soft microbots with tunable traction.Science advances · 2023Article
- Atomic Force Microscopy (AFM) Applications in Arrhythmogenic Cardiomyopathy.International journal of molecular sciences · 2022Review
- Hyperglycemia and hyperlipidemia can induce morphophysiological changes in rat cardiac cell line.Biochemistry and biophysics reports · 2021Article
- Measuring the elastic modulus of soft culture surfaces and three-dimensional hydrogels using atomic force microscopy.Nature protocols · 2021Article
- The ArabidopsisGenes · 2021Article
- Free-Standing Graphene Oxide and Carbon Nanotube Hybrid Papers with Enhanced Electrical and Mechanical Performance and Their Synergy in Polymer Laminates.International journal of molecular sciences · 2020Article
- Indenting soft samples (hydrogels and cells) with cantilevers possessing various shapes of probing tip.European biophysics journal : EBJ · 2020Article
- Mechanical Characterization for Cellular Mechanobiology: Current Trends and Future Prospects.Frontiers in bioengineering and biotechnology · 2020Review
- The head and neck cancer (HN-5) cell line properties extraction by AFM.Journal of biological engineering · 2020Article
- Increased stiffness and flow resistance of the inner wall of Schlemm's canal in glaucomatous human eyes.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Depth-Sensing Indentation as a Micro- and Nanomechanical Approach to Characterisation of Mechanical Properties of Soft, Biological, and Biomimetic Materials.Nanomaterials (Basel, Switzerland) · 2019Review
- Nonlinear Cellular Mechanical Behavior Adaptation to Substrate Mechanics Identified by Atomic Force Microscope.International journal of molecular sciences · 2018Article
- An engineered, quantifiable in vitro model for analysing the effect of proteostasis-targeting drugs on tissue physical properties.Biomaterials · 2018Article
- Investigation of fullerenol-induced changes in poroelasticity of human hepatocellular carcinoma by AFM-based creep tests.Biomechanics and modeling in mechanobiology · 2018Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Atomic force microscopy (AFM) indentation has become an important technique for quantifying the mechanical properties of live cells at nanoscale. However, determination of cell elasticity modulus from the force-displacement curves measured in the AFM indentations is not a trivial task. The present work shows that these force-displacement curves are affected by indenter-cell adhesion force, while the use of an appropriate indentation model may provide information on the cell elasticity and the work of adhesion of the cell membrane to the surface of the AFM probes. A recently proposed indentation model (Sirghi, Rossi in Appl Phys Lett 89:243118, 2006), which accounts for the effect of the adhesion force in nanoscale indentation, is applied to the AFM indentation experiments performed on live cells with pyramidal indenters. The model considers that the indentation force equilibrates the elastic force of the cell cytoskeleton and the adhesion force of the cell membrane. It is assumed that the indenter-cell contact area and the adhesion force decrease continuously during the unloading part of the indentation (peeling model). Force-displacement curves measured in indentation experiments performed with silicon nitride AFM probes with pyramidal tips on live cells (mouse fibroblast Balb/c3T3 clone A31-1-1) in physiological medium at 37 degrees C agree well with the theoretical prediction and are used to determine the cell elasticity modulus and indenter-cell work of adhesion.
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