ArticlePLoS neglected tropical diseases2008
First cultivation and characterization of Mycobacterium ulcerans from the environment.
Article in PLoS neglected tropical diseases, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 90 papers, 2 of them syntheses that pooled it.
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Who cites it
90 citing papers in PubMed, 2 syntheses or guidelines pooled it, 211 citations in OpenAlex.
- A need for null models in understanding disease transmission: the example of Mycobacterium ulcerans (Buruli ulcer disease).FEMS microbiology reviews · 2022Pooled it
- Ecology and transmission of Buruli ulcer disease: a systematic review.PLoS neglected tropical diseases · 2010Pooled it
- Cutaneous MAC infection in an immunocompetent patient: a case report confirmed by mNGS.BMC infectious diseases · 2026Article
- Comparative analysis of Buruli ulcer in Ghana and Côte d'Ivoire: A cross-sectional study.PLoS neglected tropical diseases · 2026Article
- Defining new Buruli ulcer endemic areas in urban southeastern Australia using bacterial genomics-informed possum excreta surveys.Applied and environmental microbiology · 2025Article
- Risk factors for Buruli ulcer disease in Ghana: A matched case-control study in four selected endemic districts of Eastern and Oti Regions.PLoS neglected tropical diseases · 2025Article
- Buruli ulcer surveillance in south-eastern Australian possums: Infection status, lesion mapping and internal distribution of Mycobacterium ulcerans.PLoS neglected tropical diseases · 2024Article
- A low-cost and versatile paramagnetic bead DNA extraction method forApplied and environmental microbiology · 2024Article
- Systematic review: Global host range, case fatality and detection rates ofJournal of clinical tuberculosis and other mycobacterial diseases · 2024Review
- Emergence and spread ofMicrobiology spectrum · 2024Article
- Article
- Perceived water-related risk factors of Buruli ulcer in two villages of south-central Côte d'Ivoire.PLoS neglected tropical diseases · 2022Article
- A combined field study of Buruli ulcer disease in southeast Benin proposing preventive strategies based on epidemiological, geographic, behavioural and environmental analyses.PLOS global public health · 2022Article
- Linking theOne health (Amsterdam, Netherlands) · 2021Review
- Understanding the transmission of Mycobacterium ulcerans: A step towards controlling Buruli ulcer.PLoS neglected tropical diseases · 2021Review
- Introduction of Mycobacterium ulcerans disease in the Bankim Health District of Cameroon follows damming of the Mapé River.PLoS neglected tropical diseases · 2020Article
- Tracing Mycobacterium ulcerans along an alimentary chain in Côte d'Ivoire: A one health perspective.PLoS neglected tropical diseases · 2020Article
- Translocating Mycobacterium ulcerans: An experimental model.PloS one · 2020Article
- Screening anti-infectious molecules against Mycobacterium ulcerans: A step towards decontaminating environmental specimens.PloS one · 2020Article
- DisseminatedThe American journal of tropical medicine and hygiene · 2019Article
30 more citing papers are in PubMed but not listed here.
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Authors and funding
17 authors at 5 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMycobacterium ulcerans disease, or Buruli ulcer (BU), is an indolent, necrotizing infection of skin, subcutaneous tissue and, occasionally, bones. It is the third most common human mycobacteriosis worldwide, after tuberculosis and leprosy. There is evidence that M. ulcerans is an environmental pathogen transmitted to humans from aquatic niches; however, well-characterized pure cultures of M. ulcerans from the environment have never been reported. Here we present details of the isolation and characterization of an M. ulcerans strain (00-1441) obtained from an aquatic Hemiptera (common name Water Strider, Gerris sp.) from Benin. METHODOLOGY/PRINCIPAL
findingsOne culture from a homogenate of a Gerris sp. in BACTEC became positive for IS2404, an insertion sequence with more than 200 copies in M. ulcerans. A pure culture of M. ulcerans 00-1441 was obtained on Löwenstein-Jensen medium after inoculation of BACTEC culture in mouse footpads followed by two other mouse footpad passages. The phenotypic characteristics of 00-1441 were identical to those of African M. ulcerans, including production of mycolactone A/B. The nucleotide sequence of the 5' end of 16S rRNA gene of 00-1441 was 100% identical to M. ulcerans and M. marinum, and the sequence of the 3' end was identical to that of the African type except for a single nucleotide substitution at position 1317. This mutation in M. ulcerans was recently discovered in BU patients living in the same geographic area. Various genotyping methods confirmed that strain 00-1441 has a profile identical to that of the predominant African type. Strain 00-1441 produced severe progressive infection and disease in mouse footpads with involvement of bone.
conclusionStrain 00-1441 represents the first genetically and phenotypically identified strain of M. ulcerans isolated in pure culture from the environment. This isolation supports the concept that the agent of BU is a human pathogen with an environmental niche.
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