ArticleThe Plant cell2008
Exclusion of a proton ATPase from the apical membrane is associated with cell polarity and tip growth in Nicotiana tabacum pollen tubes.
Article in The Plant cell, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
60 citing papers in PubMed, 1 synthesis or guideline pooled it, 129 citations in OpenAlex.
- Root hair growth from the pH point of view.Frontiers in plant science · 2022Pooled it
- Effect of UV-B stress on olive (Olea europaea L.) pollen tubes: A study of callose plug deposition and male germ unit integrity.Protoplasma · 2025Article
- The transcription factor PbrbZIP52 positively affects pear pollen tube longevity by promoting callose synthesis.Plant physiology · 2023Article
- Depletion plays a pivotal role in self-incompatibility, revealing a link between cellular energy status, cytosolic acidification and actin remodelling in pollen tubes.The New phytologist · 2022Article
- The legacy of kinesins in the pollen tube 30 years later.Cytoskeleton (Hoboken, N.J.) · 2022Review
- Two Is Company, but Four Is a Party-Challenges of Tetraploidization for Cell Wall Dynamics and Efficient Tip-Growth in Pollen.Plants (Basel, Switzerland) · 2021Review
- Spatiotemporal Quantification of Cytosolic pH inBio-protocol · 2021Article
- Pollen Germination and Pollen Tube Growth in Gymnosperms.Plants (Basel, Switzerland) · 2021Review
- The quest for the central players governing pollen tube growth and guidance.Plant physiology · 2021Review
- Measuring Extracellular Proton and Anionic Fluxes inBio-protocol · 2021Article
- ROS and Ions in Cell Signaling during Sexual Plant Reproduction.International journal of molecular sciences · 2020Review
- Dynamics of Pollen Activation and the Role of HPlants (Basel, Switzerland) · 2020Article
- Signalling Pinpointed to the Tip: The Complex Regulatory Network That Allows Pollen Tube Growth.Plants (Basel, Switzerland) · 2020Review
- Plasma membrane HNature communications · 2020Article
- Roles of plasma membrane proton ATPases AHA2 and AHA7 in normal growth of roots and root hairs in Arabidopsis thaliana.Physiologia plantarum · 2019Article
- Unusual Roles of Secretory SNARE SYP132 in Plasma Membrane HPlant physiology · 2019Article
- Depletion of sucrose induces changes in the tip growth mechanism of tobacco pollen tubes.Annals of botany · 2018Article
- Bioelectric signaling in regeneration: Mechanisms of ionic controls of growth and form.Developmental biology · 2018Review
- Interplay between Ions, the Cytoskeleton, and Cell Wall Properties during Tip Growth.Plant physiology · 2018Review
- Endogenous Bioelectric Signaling Networks: Exploiting Voltage Gradients for Control of Growth and Form.Annual review of biomedical engineering · 2017Review
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 3 countries.
Funding
Abstract
Polarized growth in pollen tubes results from exocytosis at the tip and is associated with conspicuous polarization of Ca(2+), H(+), K(+), and Cl(-) -fluxes. Here, we show that cell polarity in Nicotiana tabacum pollen is associated with the exclusion of a novel pollen-specific H(+)-ATPase, Nt AHA, from the growing apex. Nt AHA colocalizes with extracellular H(+) effluxes, which revert to influxes where Nt AHA is absent. Fluorescence recovery after photobleaching analysis showed that Nt AHA moves toward the apex of growing pollen tubes, suggesting that the major mechanism of insertion is not through apical exocytosis. Nt AHA mRNA is also excluded from the tip, suggesting a mechanism of polarization acting at the level of translation. Localized applications of the cation ionophore gramicidin A had no effect where Nt AHA was present but acidified the cytosol and induced reorientation of the pollen tube where Nt AHA was absent. Transgenic pollen overexpressing Nt AHA-GFP developed abnormal callose plugs accompanied by abnormal H(+) flux profiles. Furthermore, there is no net flux of H(+) in defined patches of membrane where callose plugs are to be formed. Taken together, our results suggest that proton dynamics may underlie basic mechanisms of polarity and spatial regulation in growing pollen tubes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.