ArticleProceedings of the National Academy of Sciences of the United States of America2008
Culturing of human peripheral blood cells reveals unsuspected lymphocyte responses relevant to HIV disease.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 37 citations in OpenAlex.
- Trial
- HIV-1 Tat B-cell epitope vaccination was ineffectual in preventing viral rebound after ART cessation: HIV rebound with current ART appears to be due to infection with new endogenous founder virus and not to resurgence of pre-existing Tat-dependent viremia.Human vaccines & immunotherapeutics · 2012Trial
- Changes in immune cell populations during acclimatization to high altitude.Physiological reports · 2024Article
- The in vitro treatment of mesenchymal stem cells for colorectal cancer cells.Medical oncology (Northwood, London, England) · 2023Article
- Steroid Responsiveness in Duchenne Muscular Dystrophy - Predictive Value of Epigenetic Regulator Histone Deacetylase 2.Indian journal of pediatrics · 2020Article
- Reciprocal Relationship Between HDAC2 and P-Glycoprotein/MRP-1 and Their Role in Steroid Resistance in Childhood Nephrotic Syndrome.Frontiers in pharmacology · 2019Article
- Quantitative proteomics reveals oxygen-dependent changes in neuronal mitochondria affecting function and sensitivity to rotenone.Journal of proteome research · 2013Article
- Redox regulation of Janus kinase: The elephant in the room.JAK-STAT · 2013Review
- Role of cellular iron and oxygen in the regulation of HIV-1 infection.Future virology · 2013Article
- H-Y antigen-binding B cells develop in male recipients of female hematopoietic cells and associate with chronic graft vs. host disease.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Our NIH years: a confluence of beginnings.The Journal of biological chemistry · 2013Article
- Human monocytes and macrophages differ in their mechanisms of adaptation to hypoxia.Arthritis research & therapy · 2012Article
- Glut1-mediated glucose transport regulates HIV infection.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Oxygen matters: tissue culture oxygen levels affect mitochondrial function and structure as well as responses to HIV viroproteins.Cell death & disease · 2011Article
- Diametrically opposed effects of hypoxia and oxidative stress on two viral transactivators.Virology journal · 2010Article
- Intracellular transactivation of HIV can account for the decelerating decay of virus load during drug therapy.Molecular systems biology · 2010Article
- Regulation of HIV-1 transcription at 3% versus 21% oxygen concentration.Journal of cellular physiology · 2009Article
- Zinc coordination is required for and regulates transcription activation by Epstein-Barr nuclear antigen 1.PLoS pathogens · 2009Article
- Microfluidic platform for real-time signaling analysis of multiple single T cells in parallel.Lab on a chip · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Recombinant HIV-Tat (Tat) induces extensive apoptosis in peripheral blood mononuclear cells (PBMCs) cultured in typical CO2 incubators, which are equilibrated with air (21% O2). However, as we show here, Tat apoptosis induction fails in PBMCs cultured at physiological oxygen levels (5% O2). Under these conditions, Tat induces PBMCs to divide, efficiently primes them for HIV infection, and supports virus production by the infected cells. Furthermore, Tat takes only 2 h to prime PBMCs under these conditions. In contrast, PHA/IL-2, which is widely used to prime cells for HIV infection, takes 2-3 days. These findings strongly recommend culturing primary cells at physiological oxygen levels. In addition, they suggest HIV-Tat as a key regulator of HIV disease progression.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.