ArticleInternational journal for parasitology2008
Characterization of the promoter of the Brugia malayi 12kDa small subunit ribosomal protein (RPS12) gene.
Article in International journal for parasitology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 17 citations in OpenAlex.
- Prediction pipeline for discovery of regulatory motifs associated with Brugia malayi molting.PLoS neglected tropical diseases · 2020Article
- In vivo imaging of transgenic Brugia malayi.PLoS neglected tropical diseases · 2020Article
- CRISPR/Cas9 Mutagenesis and Expression of Dominant Mutant Transgenes as Functional Genomic Approaches in Parasitic Nematodes.Frontiers in genetics · 2019Review
- Development of a toolkit for piggyBac-mediated integrative transfection of the human filarial parasite Brugia malayi.PLoS neglected tropical diseases · 2018Article
- Nucleic acid transfection and transgenesis in parasitic nematodes.Parasitology · 2012Review
- Analysis of transcriptional regulation of tetracycline responsive genes in Brugia malayi.Molecular and biochemical parasitology · 2011Article
- In vivo transfection of developmentally competent Brugia malayi infective larvae.International journal for parasitology · 2011Article
- The role of polymorphisms in the spliced leader addition domain in determining promoter activity in Brugia malayi.Molecular and biochemical parasitology · 2011Article
- Molecular evidence for a functional ecdysone signaling system in Brugia malayi.PLoS neglected tropical diseases · 2010Article
- The role of local secondary structure in the function of the trans-splicing motif of Brugia malayi.Molecular and biochemical parasitology · 2010Article
- Functional analysis of putative operons in Brugia malayi.International journal for parasitology · 2010Article
- Helminth genomics: The implications for human health.PLoS neglected tropical diseases · 2009Review
- The splice leader addition domain represents an essential conserved motif for heterologous gene expression in B. malayi.Molecular and biochemical parasitology · 2009Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Unravelling gene regulatory mechanisms in human filarial parasites will require an understanding of their basic promoter structure. Only a single promoter from a human filarial parasite has been characterised in detail, the 70 kDa heat shock promoter of Brugia malayi (BmHSP70). This promoter was found to lack features found in a typical eukaryotic promoter. To determine if this was unique to the BmHSP70 promoter, a detailed analysis was undertaken of the promoter for the B. malayi small subunit 12 kDa ribosomal protein (BmRPS12) gene. The BmRPS12 promoter contained a unique tandem repeat structure. Deletion of these repeats resulted in the loss of 80% of promoter activity. Block replacement mutagenesis identified five regions outside the repeat which were essential for promoter activity. No predicted binding sites for proteins that normally associate with the typical eukaryotic core promoter domains were found in the essential domains or the repeat region. However, the repeat region contained many putative binding sites for GATAA transcription factor family proteins. Of 20 upstream domains of other ribosomal protein genes, one contained a repeat structure similar to that found in the BmRPS12 promoter, and the majority encoded putative GATAA transcription factor binding sites. This study demonstrates that the BmRPS12 promoter, like the BmHSP70 promoter, is distinct from a typical eukaryotic promoter.
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