ArticleMolecular microbiology2008
The metabolic activity of Mycobacterium tuberculosis, assessed by use of a novel inducible GFP expression system, correlates with its capacity to inhibit phagosomal maturation and acidification in human macrophages.
Article in Molecular microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 36 citations in OpenAlex.
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- DMN-Tre Labeling for Detection and High-Content Screening of Compounds against Intracellular Mycobacteria.ACS omega · 2020Article
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- Autophagy induction targeting mTORC1 enhances Mycobacterium tuberculosis replication in HIV co-infected human macrophages.Scientific reports · 2016Article
- Output-driven feedback system control platform optimizes combinatorial therapy of tuberculosis using a macrophage cell culture model.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Article
- Mycobacterium tuberculosis exploits asparagine to assimilate nitrogen and resist acid stress during infection.PLoS pathogens · 2014Article
- Regulated Expression Systems for Mycobacteria and Their Applications.Microbiology spectrum · 2014Article
- Development of a new generation of vectors for gene expression, gene replacement, and protein-protein interaction studies in mycobacteria.Applied and environmental microbiology · 2013Article
- Uptake and persistence of Mycobacterium avium subsp. paratuberculosis in human monocytes.Infection and immunity · 2012Article
- The Mycobacterium tuberculosis SecA2 system subverts phagosome maturation to promote growth in macrophages.Infection and immunity · 2012Article
- Discovery and characterization of a unique mycobacterial heme acquisition system.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Importance of phagosomal functionality for growth restriction of Mycobacterium tuberculosis in primary human macrophages.Journal of innate immunity · 2011Article
- Intracellular Mycobacterium avium intersect transferrin in the Rab11(+) recycling endocytic pathway and avoid lipocalin 2 trafficking to the lysosomal pathway.The Journal of infectious diseases · 2010Article
- The Mycobacterium bovis bacille Calmette-Guerin phagosome proteome.Molecular & cellular proteomics : MCP · 2010Article
- Autophagy gene variant IRGM -261T contributes to protection from tuberculosis caused by Mycobacterium tuberculosis but not by M. africanum strains.PLoS pathogens · 2009Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Mycobacterium tuberculosis generally reside in phagosomes within human macrophages that resist maturation and acidification, but exhibit significant heterogeneity. In this study we have constructed an IPTG-inducible GFP expression system in M. tuberculosis to assess the relationship between the metabolic status of M. tuberculosis and the degree of phagosomal maturation. Using these recombinant bacteria, we have found that, in human macrophages, M. tuberculosis that respond to IPTG with expression of GFP fluorescence, and hence are metabolically active, reside in non-acidified phagosomes that have not fused with Texas red dextran pre-labelled lysosomes. In contrast, M. tuberculosis that fail to express GFP in response to IPTG, and hence are metabolically inactive, reside within acidified phagosomes that have fused with Texas red dextran labelled lysosomes. These studies demonstrate that metabolic activity of M. tuberculosis correlates strongly with phagosomal maturation and that the inducible GFP expression system is useful for assessing metabolic activity of intracellular M. tuberculosis.
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Registered trials
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