ArticleMolecular microbiology2008
TtsI regulates symbiotic genes in Rhizobium species NGR234 by binding to tts boxes.
Article in Molecular microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 73 citations in OpenAlex.
- Phosphorylation of rhizobial effector NopZ by soybean NORK promotes association with NENA and enhances nodulation.Nature communications · 2026Article
- Diversity of bradyrhizobial T3SS systems and their roles in symbiosis with peanut (Applied and environmental microbiology · 2025Article
- NopP2 effector of Bradyrhizobium elkanii USDA61 is a determinant of nodulation in Vigna radiata cultivars.Scientific reports · 2024Article
- Calcium Induces the Cleavage of NopA and Regulates the Expression of Nodulation Genes and Secretion of T3SS Effectors inInternational journal of molecular sciences · 2024Article
- Secretory molecules from secretion systems fine-tune the host-beneficial bacteria (PGPRs) interaction.Frontiers in microbiology · 2024Review
- The Rhizobial Type 3 Secretion System: The Dr. Jekyll and Mr. Hyde in the Rhizobium-Legume Symbiosis.International journal of molecular sciences · 2022Review
- The NEL Family of Bacterial E3 Ubiquitin Ligases.International journal of molecular sciences · 2022Review
- Bridging the Gap: Type III Secretion Systems in Plant-Beneficial Bacteria.Microorganisms · 2022Article
- Rhizobia use a pathogenic-like effector to hijack leguminous nodulation signalling.Scientific reports · 2021Article
- Phylogenetic distribution and evolutionary dynamics ofMicrobial genomics · 2020Article
- Article
- Lotus Accessions Possess Multiple Checkpoints Triggered by Different Type III Secretion System Effectors of the Wide-Host-Range Symbiont Bradyrhizobium elkanii USDA61.Microbes and environments · 2020Article
- The rhizobial type III effector ErnA confers the ability to form nodules in legumes.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- QTL analysis of nodule traits and the identification of loci interacting with the type III secretion system in soybean.Molecular genetics and genomics : MGG · 2019Article
- High-Throughput Mass Spectrometric Analysis of the Whole Proteome and Secretome FromFrontiers in microbiology · 2019Article
- Variation in bradyrhizobial NopP effector determines symbiotic incompatibility with Rj2-soybeans via effector-triggered immunity.Nature communications · 2018Article
- InnB, a Novel Type III Effector ofFrontiers in microbiology · 2018Article
- Identification of Bradyrhizobium elkanii Genes Involved in Incompatibility with Vigna radiata.Genes · 2017Article
- The Sinorhizobium (Ensifer) fredii HH103 Nodulation Outer Protein NopI Is a Determinant for Efficient Nodulation of Soybean and Cowpea Plants.Applied and environmental microbiology · 2017Article
- Rhizobium-legume symbiosis in the absence of Nod factors: two possible scenarios with or without the T3SS.The ISME journal · 2016Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Infection of legumes by Rhizobium sp. NGR234 and subsequent development of nitrogen-fixing nodules are dependent on the coordinated actions of Nod factors, proteins secreted by a type III secretion system (T3SS) and modifications to surface polysaccharides. The production of these signal molecules is dependent on plant flavonoids which trigger a regulatory cascade controlled by the transcriptional activators NodD1, NodD2, SyrM2 and TtsI. TtsI is known to control the genes responsible for T3SS function and synthesis of a symbiotically important rhamnose-rich lipo-polysaccharide, most probably by binding to cis elements termed tts boxes. Eleven tts boxes were identified in the promoter regions of target genes on the symbiotic plasmid of NGR234. Expression profiles of lacZ fusions to these tts boxes showed that they are part of a TtsI-dependent regulon induced by plant-derived flavonoids. TtsI was purified and demonstrated to bind directly to two of these tts boxes. DNase I footprinting revealed that TtsI occupied not only the tts box consensus sequence, but also upstream and downstream regions in a concentration-dependent manner. Highly conserved bases of the consensus tts box were mutated and, although TtsI binding was still observed in vitro, gfp fusions were no longer transcribed in vivo. Random mutagenesis of a tts box-containing promoter revealed more nucleotides critical for transcriptional activity outside of the consensus.
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