ArticleAntimicrobial agents and chemotherapy2008
The efflux pump inhibitor reserpine selects multidrug-resistant Streptococcus pneumoniae strains that overexpress the ABC transporters PatA and PatB.
Article in Antimicrobial agents and chemotherapy, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
41 citing papers in PubMed, 102 citations in OpenAlex.
- Antibiotic resistance mediated by gene amplifications.npj antimicrobials and resistance · 2024Review
- Thymoquinone' potent impairment of multidrug-resistant Staphylococcus aureus NorA efflux pump activity.Scientific reports · 2024Article
- P-type ATPase zinc transporter Rv3270 ofMicrobiology (Reading, England) · 2024Article
- Article
- Putative Role of an ABC Efflux System inAntibiotics (Basel, Switzerland) · 2023Article
- Role of PatAB Transporter in Efflux of Levofloxacin inAntibiotics (Basel, Switzerland) · 2022Article
- Susceptibility Tests and Predictions of Transporter Profile inMicroorganisms · 2022Article
- Drug-dependent inhibition of nucleotide hydrolysis in the heterodimeric ABC multidrug transporter PatAB from Streptococcus pneumoniae.The FEBS journal · 2022Article
- Phenotypic Adaptation to Antiseptics and Effects on Biofilm Formation Capacity and Antibiotic Resistance in Clinical Isolates of Early Colonizers in Dental Plaque.Antibiotics (Basel, Switzerland) · 2022Article
- A CFrontiers in microbiology · 2022Article
- Advances in adjunct therapy against tuberculosis: Deciphering the emerging role of phytochemicals.MedComm · 2021Review
- Selective Targeting of Human and Animal Pathogens of theInternational journal of molecular sciences · 2021Article
- Bacterial Resistance to Antimicrobial Agents.Antibiotics (Basel, Switzerland) · 2021Review
- Plant-derived secondary metabolites as the main source of efflux pump inhibitors and methods for identification.Journal of pharmaceutical analysis · 2020Review
- In Vitro Evaluation of the Antimicrobial and Immunomodulatory Activity of Culinary Herb Essential Oils as Potential Perioceutics.Antibiotics (Basel, Switzerland) · 2020Article
- Alkaloid extracts from Combretum zeyheri inhibit the growth of Mycobacterium smegmatis.BMC complementary and alternative medicine · 2017Article
- Upregulation of the PatAB Transporter Confers Fluoroquinolone Resistance toFrontiers in microbiology · 2017Article
- Antimicrobial Activity of Zabofloxacin against Clinically Isolated Streptococcus pneumoniae.Molecules (Basel, Switzerland) · 2016Article
- Sequence-Specific Targeting of Bacterial Resistance Genes Increases Antibiotic Efficacy.PLoS biology · 2016Article
- Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
One way to combat multidrug-resistant microorganisms is the use of efflux pump inhibitors (EPIs). Spontaneous mutants resistant to the EPI reserpine selected from Streptococcus pneumoniae NCTC 7465 and R6 at a frequency suggestive of a single mutational event were also multidrug resistant. No mutations in pmrA (which encodes the efflux protein PmrA) were detected, and the expression of pmrA was unaltered in all mutants. In the reserpine-resistant multidrug-resistant mutants, the overexpression of both patA and patB, which encode ABC transporters, was associated with accumulation of low concentrations of antibiotics and dyes. The addition of sodium orthovanadate, an inhibitor of ABC efflux pumps, or the insertional inactivation of either gene restored wild-type antibiotic susceptibility and wild-type levels of accumulation. Only when patA was insertionally inactivated were both multidrug resistance and reserpine resistance lost. Strains in which patA was insertionally inactivated grew significantly more slowly than the wild type. These data indicate that the overexpression of both patA and patB confers multidrug resistance in S. pneumoniae but that only patA is involved in reserpine resistance. The selection of reserpine-resistant multidrug-resistant pneumococci has implications for analogous systems in other bacteria or in cancer.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.