ArticleMolecular and cellular biology2008
The silent information regulator 3 protein, SIR3p, binds to chromatin fibers and assembles a hypercondensed chromatin architecture in the presence of salt.
Article in Molecular and cellular biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 42 citations in OpenAlex.
- Transcriptional silencing in Saccharomyces cerevisiae: known unknowns.Epigenetics & chromatin · 2024Review
- Sir3 mediates long-range chromosome interactions in budding yeast.Genome research · 2021Article
- SIR proteins create compact heterochromatin fibers.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- The elongation factor Spn1 is a multi-functional chromatin binding protein.Nucleic acids research · 2018Article
- The 10-nm chromatin fiber and its relationship to interphase chromosome organization.Biochemical Society transactions · 2018Review
- The Nuts and Bolts of Transcriptionally Silent Chromatin in Saccharomyces cerevisiae.Genetics · 2016Review
- Nucleosomal arrays self-assemble into supramolecular globular structures lacking 30-nm fibers.The EMBO journal · 2016Article
- Nucleosome structure and function.Chemical reviews · 2015Review
- Solution-state conformation and stoichiometry of yeast Sir3 heterochromatin fibres.Nature communications · 2014Article
- Assembly of nucleosomal arrays from recombinant core histones and nucleosome positioning DNA.Journal of visualized experiments : JoVE · 2013Article
- Detection of an altered heterochromatin structure in the absence of the nucleotide excision repair protein Rad4 in Saccharomyces cerevisiae.Cell cycle (Georgetown, Tex.) · 2013Article
- The role of the nucleosome acidic patch in modulating higher order chromatin structure.Journal of the Royal Society, Interface · 2013Review
- Dimerization of Sir3 via its C-terminal winged helix domain is essential for yeast heterochromatin formation.The EMBO journal · 2013Article
- Structure and function in the budding yeast nucleus.Genetics · 2012Review
- Short nucleosome repeats impose rotational modulations on chromatin fibre folding.The EMBO journal · 2012Article
- Chromatin modification by PSC occurs at one PSC per nucleosome and does not require the acidic patch of histone H2A.PloS one · 2012Article
- Structural basis of silencing: Sir3 BAH domain in complex with a nucleosome at 3.0 Å resolution.Science (New York, N.Y.) · 2011Article
- Chromatin higher-order structures and gene regulation.Current opinion in genetics & development · 2011Review
- Clustering heterochromatin: Sir3 promotes telomere clustering independently of silencing in yeast.The Journal of cell biology · 2011Article
- Chromosome arm length and nuclear constraints determine the dynamic relationship of yeast subtelomeres.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The telomeres and mating-type loci of budding yeast adopt a condensed, heterochromatin-like state through recruitment of the silent information regulator (SIR) proteins SIR2p, SIR3p, and SIR4p. In this study we characterize the chromatin binding determinants of recombinant SIR3p and identify how SIR3p mediates chromatin fiber condensation in vitro. Purified full-length SIR3p was incubated with naked DNA, nucleosome core particles, or defined nucleosomal arrays, and the resulting complexes were analyzed by electrophoretic shift assays, sedimentation velocity, and electron microscopy. SIR3p bound avidly to all three types of templates. SIR3p loading onto its nucleosomal sites in chromatin produced thickened condensed fibers that retained a beaded morphology. At higher SIR3p concentrations, individual nucleosomal arrays formed oligomeric suprastructures bridged by SIR3p oligomers. When condensed SIR3p-bound chromatin fibers were incubated in Mg(2+), they folded and oligomerized even further to produce hypercondensed higher-order chromatin structures. Collectively, these results define how SIR3p may function as a chromatin architectural protein and provide new insight into the interplay between endogenous and protein-mediated chromatin fiber condensation pathways.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.