ArticleJournal of lipid research2008
An analysis of the role of a retroendocytosis pathway in ABCA1-mediated cholesterol efflux from macrophages.
Article in Journal of lipid research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 58 citations in OpenAlex.
- High density lipoproteins and extracellular vesicles-distinct but overlapping circulating particles and their role in atherosclerosis.Frontiers in cardiovascular medicine · 2026Review
- Molecular mechanisms for ABCA1-mediated cholesterol efflux.Cell cycle (Georgetown, Tex.) · 2022Review
- Is ABCA1 a lipid transfer protein?Journal of lipid research · 2018Review
- Exposure to High Glucose Concentration Decreases Cell Surface ABCA1 and HDL Biogenesis in Hepatocytes.Journal of atherosclerosis and thrombosis · 2017Article
- ABCA1 (ATP-Binding Cassette Transporter A1) Mediates ApoA-I (Apolipoprotein A-I) and ApoA-I Mimetic Peptide Mobilization of Extracellular Cholesterol Microdomains Deposited by Macrophages.Arteriosclerosis, thrombosis, and vascular biology · 2016Article
- Small GTPase ARF6 Regulates Endocytic Pathway Leading to Degradation of ATP-Binding Cassette Transporter A1.Arteriosclerosis, thrombosis, and vascular biology · 2016Article
- The unsolved mystery of apoA-I recycling in adipocyte.Lipids in health and disease · 2016Review
- ABCA1-dependent sterol release: sterol molecule specificity and potential membrane domain for HDL biogenesis.Journal of lipid research · 2016Article
- Molecular mechanisms of cellular cholesterol efflux.The Journal of biological chemistry · 2014Review
- Factors controlling nascent high-density lipoprotein particle heterogeneity: ATP-binding cassette transporter A1 activity and cell lipid and apolipoprotein AI availability.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2013Article
- The interaction of ApoA-I and ABCA1 triggers signal transduction pathways to mediate efflux of cellular lipids.Molecular medicine (Cambridge, Mass.) · 2012Review
- Regulation of ABCA1 functions by signaling pathways.Biochimica et biophysica acta · 2012Review
- Cytoskeleton disruption in J774 macrophages: consequences for lipid droplet formation and cholesterol flux.Biochimica et biophysica acta · 2012Article
- A novel compound inhibits reconstituted high-density lipoprotein assembly and blocks nascent high-density lipoprotein biogenesis downstream of apolipoprotein AI binding to ATP-binding cassette transporter A1-expressing cells.Arteriosclerosis, thrombosis, and vascular biology · 2011Article
- Update on HDL receptors and cellular cholesterol transport.Current atherosclerosis reports · 2011Review
- The β-subunit of ATP synthase is involved in cellular uptake and resecretion of apoA-I but does not control apoA-I-induced lipid efflux in adipocytes.Molecular and cellular biochemistry · 2011Article
- Caveolin-1-mediated apolipoprotein A-I membrane binding sites are not required for cholesterol efflux.PloS one · 2011Article
- Reverse cholesterol transport: from classical view to new insights.World journal of gastroenterology · 2010Review
- Influence of apolipoprotein (Apo) A-I structure on nascent high density lipoprotein (HDL) particle size distribution.The Journal of biological chemistry · 2010Article
- ABC transporters, atherosclerosis and inflammation.Atherosclerosis · 2010Review
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
The ATP binding cassette transporter A-1 (ABCA1) is critical for apolipoprotein-mediated cholesterol efflux, an important mechanism employed by macrophages to avoid becoming lipid-laden foam cells, the hallmark of early atherosclerotic lesions. It has been proposed that lipid-free apolipoprotein A-I (apoA-I) enters the cell and is resecreted as a lipidated particle via a retroendocytosis pathway during ABCA1-mediated cholesterol efflux from macrophages. To determine the functional importance of such a pathway, confocal microscopy was used to characterize the internalization of a fully functional apoA-I cysteine mutant containing a thiol-reactive fluorescent probe in cultured macrophages. ApoA-I was also endogenously labeled with (35)S-methionine to quantify cellular uptake and to determine the metabolic fate of the internalized protein. It was found that apoA-I was specifically taken inside macrophages and that a small amount of intact apoA-I was resecreted from the cells. However, a majority of the label that reappeared in the media was degraded. We estimate that the mass of apoA-I retroendocytosed is not sufficient to account for the HDL produced by the cholesterol efflux reaction. Furthermore, we have demonstrated that lipid-free apoA-I-mediated cholesterol efflux from macrophages can be pharmacologically uncoupled from apoA-I internalization into cells. On the basis these findings, we present a model in which the ABCA1-mediated lipid transfer process occurs primarily at the membrane surface in macrophages, but still accounts for the observed specific internalization of apoA-I.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.