ArticlePlant physiology2008
Characterization of cold-responsive extracellular chitinase in bromegrass cell cultures and its relationship to antifreeze activity.
Article in Plant physiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 57 citations in OpenAlex.
- Temperature variability and other climatic attributes linked to genomic features in the lichen-forming fungal genus Umbilicaria.BMC biology · 2025Article
- Genome-Wide Identification and Expression Analysis of the GH19 Chitinase Gene Family in Sea Island Cotton.Current issues in molecular biology · 2025Article
- Genome-wide identification of chitinase gene family in Hordeum vulgare: insights into stress response mechanisms and evolutionary dynamics.BMC plant biology · 2025Article
- Biochemical and transcriptomic profiling analysis of drought tolerant related genes in ML 82-2 and ML 125-2 rice mutant lines.Biotechnologia · 2025Article
- Molecular Mechanisms Underlying Freezing Tolerance in Plants: Implications for Cryopreservation.International journal of molecular sciences · 2024Review
- Genome-Wide Identification and Expression Analysis of Chitinase Genes in Watermelon under Abiotic Stimuli andInternational journal of molecular sciences · 2024Article
- Shotgun proteomics of quinoa seeds reveals chitinases enrichment under rainfed conditions.Scientific reports · 2023Article
- Up-regulation of a stress-responsive endochitinase VvChit-IV in grapevine cell cultures improves in vitro stress tolerance.Protoplasma · 2022Article
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- TALEN-BasedFrontiers in plant science · 2021Article
- A NaFrontiers in plant science · 2020Article
- Genome-Wide Identification and Expression Analyses of the Chitinases under Cold and Osmotic stress inGenes · 2019Article
- Out in the Cold: Identification of Genomic Regions Associated With Cold Tolerance in the Biocontrol FungusFrontiers in microbiology · 2018Article
- Ice-Binding Proteins in Plants.Frontiers in plant science · 2017Review
- Antifreeze proteins enable plants to survive in freezing conditions.Journal of biosciences · 2014Review
- Refolding of β-stranded class I chitinases of Hippophae rhamnoides enhances the antifreeze activity during cold acclimation.PloS one · 2014Article
- Structural prediction of a novel chitinase from the psychrophilic Glaciozyma antarctica PI12 and an analysis of its structural properties and function.Journal of computer-aided molecular design · 2012Article
- Molecular cloning, expression and biochemical characterisation of a cold-adapted novel recombinant chitinase from Glaciozyma antarctica PI12.Microbial cell factories · 2011Article
- Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A cold-responsive chitinase gene, BiCHT1, was isolated from bromegrass (Bromus inermis) 'Manchar' suspension cells. BiCHT1 messenger RNA was detected at low levels in nonstressed bromegrass cells, whereas its accumulation was induced by incubation at 10 degrees C and 4 degrees C as detected by northern- and western-blot analyses. BiCHT1 was highly homologous to rye CHT9, known to encode an antifreeze protein. BiCHT1 was overexpressed in Escherichia coli and bromegrass cells using genetic transformation procedures. BiCHT1 products expressed in both systems had chitinase activity, but the expressed proteins did not affect the growth of ice crystals in any conditions tested. Besides cold stress, the expression of the BiCHT1 gene was up-regulated by exposure to 35 degrees C, but not by salt or osmotic stress, abscisic acid, or ethephon. BiCHT1 messenger RNA did not accumulate in response to methyl jasmonate and salicylic acid, but was slightly increased by prolonged culture at 25 degrees C and only transiently by chitin. Antifreeze activity detected in the culture medium was induced at 4 degrees C but only slightly at 10 degrees C. It was also induced by ethephon treatment, but not by abscisic acid, chitin, or prolonged incubation at 25 degrees C. The results of transgenics and expression analyses suggest that the BiCHT1 product is a major protein with chitinase activity secreted in the medium of cold-treated cells and is unlikely to be responsible for the antifreeze activity detected in the culture medium.
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