ArticleApplied and environmental microbiology2008
Comparison of methods for processing drinking water samples for the isolation of Mycobacterium avium and Mycobacterium intracellulare.
Article in Applied and environmental microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 53 citations in OpenAlex.
- Source-to-tap investigation of the occurrence of nontuberculous mycobacteria in a full-scale chloraminated drinking water system.Applied and environmental microbiology · 2024Article
- Genital mycobacteriosis caused by Mycobacterium marinum detected in two captive sharks by peptide nucleic acid-fluorescence in situ hybridization.Journal of fish diseases · 2023Article
- A New Culture Method for the Detection of Non-Tuberculous Mycobacteria in Water Samples from Heater-Cooler Units and Extracorporeal Membrane Oxygenation Machines.International journal of environmental research and public health · 2022Article
- Occurrence revisited: Mycobacterium avium and Mycobacterium intracellulare in potable water in the USA.Applied microbiology and biotechnology · 2022Article
- Comparison between the recovery rate of three concentration protocols of water samples intended for analysis by Molecular Biology: Membrane filtration, filtration on gauze pad and centrifugation.Saudi journal of biological sciences · 2022Article
- Validation of a Novel Diagnostic Approach Combining the VersaTREK™ System for Recovery and Real-Time PCR for the Identification ofMicroorganisms · 2021Article
- Environmental risk factors associated with pulmonary isolation of nontuberculous mycobacteria, a population-based study in the southeastern United States.The Science of the total environment · 2021Article
- A comprehensive evaluation of monochloramine disinfection on water quality, Legionella and other important microorganisms in a hospital.Water research · 2021Article
- Species diversity and molecular analysis of opportunistic Mycobacterium, Nocardia and Rhodococcus isolated from the hospital environment in a developing country, a potential resources for nosocomial infection.Genes and environment : the official journal of the Japanese Environmental Mutagen Society · 2021Article
- Biodegradation of polycyclic aromatic hydrocarbons, phenol and sodium sulfate by Nocardia species isolated and characterized from Iranian ecosystems.Scientific reports · 2020Article
- Recovery of Non-tuberculous Mycobacteria from Water is Influenced by Phenotypic Characteristics and Decontamination Methods.Current microbiology · 2020Review
- The sporadic nature of Legionella pneumophila, Legionella pneumophila Sg1 and Mycobacterium avium occurrence within residences and office buildings across 36 states in the United States.Journal of applied microbiology · 2019Article
- Effect of Cetylpyridinium Chloride (CPC) on Colony Formation of Common Nontuberculous Mycobacteria.Pathogens (Basel, Switzerland) · 2018Article
- Evaluation of six decontamination procedures for isolation of Mycobacterium avium complex from avian feces.PloS one · 2018Article
- Methodological approaches for monitoring opportunistic pathogens in premise plumbing: A review.Water research · 2017Review
- Epidemiology and Ecology of Opportunistic Premise Plumbing Pathogens: Legionella pneumophila, Mycobacterium avium, and Pseudomonas aeruginosa.Environmental health perspectives · 2015Review
- Isolation and molecular identification of biodegrading Mycobacteria from water supplies of Iranian hospitals.Iranian journal of microbiology · 2014Article
- Occurrence of potentially pathogenic nontuberculous mycobacteria in Mexican household potable water: a pilot study.BMC research notes · 2013Article
- Isolation of nontuberculous mycobacteria (NTM) from household water and shower aerosols in patients with pulmonary disease caused by NTM.Journal of clinical microbiology · 2013Article
- Absence of Mycobacterium intracellulare and presence of Mycobacterium chimaera in household water and biofilm samples of patients in the United States with Mycobacterium avium complex respiratory disease.Journal of clinical microbiology · 2013Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Several protocols for isolation of mycobacteria from water exist, but there is no established standard method. This study compared methods of processing potable water samples for the isolation of Mycobacterium avium and Mycobacterium intracellulare using spiked sterilized water and tap water decontaminated using 0.005% cetylpyridinium chloride (CPC). Samples were concentrated by centrifugation or filtration and inoculated onto Middlebrook 7H10 and 7H11 plates and Lowenstein-Jensen slants and into mycobacterial growth indicator tubes with or without polymyxin, azlocillin, nalidixic acid, trimethoprim, and amphotericin B. The solid media were incubated at 32 degrees C, at 35 degrees C, and at 35 degrees C with CO(2) and read weekly. The results suggest that filtration of water for the isolation of mycobacteria is a more sensitive method for concentration than centrifugation. The addition of sodium thiosulfate may not be necessary and may reduce the yield. Middlebrook M7H10 and 7H11 were equally sensitive culture media. CPC decontamination, while effective for reducing growth of contaminants, also significantly reduces mycobacterial numbers. There was no difference at 3 weeks between the different incubation temperatures.
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