ArticleArchives of biochemistry and biophysics2008
Suppression of metabolic defects of yeast isocitrate dehydrogenase and aconitase mutants by loss of citrate synthase.
Article in Archives of biochemistry and biophysics, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 22 citations in OpenAlex.
- Article
- Mitochondrial Biogenesis Is Positively Regulated by Casein Kinase I Hrr25 Through Phosphorylation of Puf3 inGenetics · 2020Article
- Cardiolipin-deficient cells depend on anaplerotic pathways to ameliorate defective TCA cycle function.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2019Article
- Cancer-associated isocitrate dehydrogenase mutations induce mitochondrial DNA instability.Human molecular genetics · 2016Article
- ATP citrate lyase mediated cytosolic acetyl-CoA biosynthesis increases mevalonate production in Saccharomyces cerevisiae.Microbial cell factories · 2016Article
- Mitochondrial DNA instability in cells lacking aconitase correlates with iron citrate toxicity.Oxidative medicine and cellular longevity · 2013Article
- The fungal α-aminoadipate pathway for lysine biosynthesis requires two enzymes of the aconitase family for the isomerization of homocitrate to homoisocitrate.Molecular microbiology · 2012Article
- Ligand binding and structural changes associated with allostery in yeast NAD(+)-specific isocitrate dehydrogenase.Archives of biochemistry and biophysics · 2012Review
- Deletion of the aconitase gene in Corynebacterium glutamicum causes strong selection pressure for secondary mutations inactivating citrate synthase.Journal of bacteriology · 2011Article
- Effects of excess succinate and retrograde control of metabolite accumulation in yeast tricarboxylic cycle mutants.The Journal of biological chemistry · 2011Article
- Large scale physiological readjustment during growth enables rapid, comprehensive and inexpensive systems analysis.BMC systems biology · 2010Article
- Deletion of citrate synthase restores growth of Sinorhizobium meliloti 1021 aconitase mutants.Journal of bacteriology · 2009Article
- Disulfide bond formation in yeast NAD+-specific isocitrate dehydrogenase.Biochemistry · 2009Article
- Redox responses in yeast to acetate as the carbon source.Archives of biochemistry and biophysics · 2009Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Yeast mutants lacking mitochondrial NAD(+)-specific isocitrate dehydrogenase (idhDelta) or aconitase (aco1Delta) were found to share several growth phenotypes as well as patterns of specific protein expression that differed from the parental strain. These shared properties of idhDelta and aco1Delta strains were eliminated or moderated by co-disruption of the CIT1 gene encoding mitochondrial citrate synthase. Gas chromatography/mass spectrometry analyses indicated a particularly dramatic increase in cellular citrate levels in idhDelta and aco1Delta strains, whereas citrate levels were substantially lower in idhDeltacit1Delta and aco1Deltacit1Delta strains. Exogenous addition of citrate to parental strain cultures partially recapitulated effects of high endogenous levels of citrate in idhDelta and aco1Delta strains. Finally, effects of elevated cellular citrate in idhDelta and aco1Delta mutant strains were partially alleviated by addition of iron or by an increase in pH of the growth medium, suggesting that detrimental effects of citrate are due to elevated levels of the ionized form of this metabolite.
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