ArticleMolecular and biochemical parasitology2008
Release of metabolic enzymes by Giardia in response to interaction with intestinal epithelial cells.
Article in Molecular and biochemical parasitology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
66 citing papers in PubMed, 169 citations in OpenAlex.
- Pathogenicity and virulence ofVirulence · 2026Review
- Iron Regulatory Mechanism IRE/IRP-like in Two Protozoa of Importance to Human Health,Pathogens (Basel, Switzerland) · 2026Review
- Arginine metabolism has a pivotal function for the encystation of Giardia duodenalis.PLoS pathogens · 2026Article
- Differentiating Canine Chronic Inflammatory Enteropathies Using Faecal Amino Acid Profiles: Potential and Limitations.Animals : an open access journal from MDPI · 2025Article
- A core UPS molecular complement implicates unique endocytic compartments at the parasite-host interface inVirulence · 2023Article
- ROS-AMPK/mTOR-dependent enterocyte autophagy is involved in the regulation ofFrontiers in immunology · 2023Article
- Specific TLR-mediated HSP70 activation plays a potential role in host defense against the intestinal parasiteFrontiers in microbiology · 2023Article
- Application of Proteomics to the Study of the Therapeutics and Pathogenicity ofDiagnostics (Basel, Switzerland) · 2022Review
- The Cysteine Protease Giardipain-1 fromInternational journal of molecular sciences · 2022Article
- The Giardial Arginine Deiminase Participates inInternational journal of molecular sciences · 2022Article
- Conserved metabolic enzymes as vaccine antigens for giardiasis.PLoS neglected tropical diseases · 2022Article
- Article
- Giardial lipid rafts share virulence factors with secreted vesicles and participate in parasitic infection in mice.Frontiers in cellular and infection microbiology · 2022Article
- A Complementary Herbal Product for Controlling Giardiasis.Antibiotics (Basel, Switzerland) · 2021Article
- Enzymology of the pathway for ATP production by arginine breakdown.The FEBS journal · 2021Article
- Giardia duodenalis Virulence - "To Be, or Not To Be".Current tropical medicine reports · 2021Review
- Serglycin-Deficiency Causes Reduced Weight Gain and Changed Intestinal Cytokine Responses in Mice Infected WithFrontiers in immunology · 2021Article
- Responses of Mast Cells to Pathogens: Beneficial and Detrimental Roles.Frontiers in immunology · 2021Review
- The Road Less Traveled? Unconventional Protein Secretion at Parasite-Host Interfaces.Frontiers in cell and developmental biology · 2021Review
- Proteomic Insights into the Biology of the Most Important Foodborne Parasites in Europe.Foods (Basel, Switzerland) · 2020Review
6 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 5 institutions in 4 countries.
Funding
Abstract
Giardia lamblia, an important cause of diarrheal disease, resides in the small intestinal lumen in close apposition to epithelial cells. Since the disease mechanisms underlying giardiasis are poorly understood, elucidating the specific interactions of the parasite with the host epithelium is likely to provide clues to understanding the pathogenesis. Here we tested the hypothesis that contact of Giardia lamblia with intestinal epithelial cells might lead to release of specific proteins. Using established co-culture models, intestinal ligated loops and a proteomics approach, we identified three G. lamblia proteins (arginine deiminase, ornithine carbamoyl transferase and enolase), previously recognized as immunodominant antigens during acute giardiasis. Release was stimulated by cell-cell interactions, since only small amounts of arginine deiminase and enolase were detected in the medium after culturing of G. lamblia alone. The secreted G. lamblia proteins were localized to the cytoplasm and the inside of the plasma membrane of trophozoites. Furthermore, in vitro studies with recombinant arginine deiminase showed that the secreted Giardia proteins can disable host innate immune factors such as nitric oxide production. These results indicate that contact of Giardia with epithelial cells triggers metabolic enzyme release, which might facilitate effective colonization of the human small intestine.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.