ArticleAnalytical chemistry2008
96-well polycarbonate-based microfluidic titer plate for high-throughput purification of DNA and RNA.
Article in Analytical chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 74 citations in OpenAlex.
- An investigation into simplifying total RNA extraction with minimal equipment using a low volume, electrokinetically driven microfluidic protocol.Biomicrofluidics · 2022Article
- Microfluidic Technologies for cfDNA Isolation and Analysis.Micromachines · 2019Review
- Microfluidic-based solid phase extraction of cell free DNA.Lab on a chip · 2018Article
- Dimethyl adipimidate/Thin film Sample processing (DTS); A simple, low-cost, and versatile nucleic acid extraction assay for downstream analysis.Scientific reports · 2015Article
- Parallel RNA extraction using magnetic beads and a droplet array.Lab on a chip · 2015Article
- Simultaneously sizing and quantitating zeptomole-level DNA at high throughput in free solution.Chemistry (Weinheim an der Bergstrasse, Germany) · 2014Article
- HIV viral RNA extraction in wax immiscible filtration assisted by surface tension (IFAST) devices.The Journal of molecular diagnostics : JMD · 2014Article
- Solid-phase extraction and purification of membrane proteins using a UV-modified PMMA microfluidic bioaffinity μSPE device.The Analyst · 2014Article
- Streamlining gene expression analysis: integration of co-culture and mRNA purification.Integrative biology : quantitative biosciences from nano to macro · 2014Article
- Selective nucleic acid removal via exclusion (SNARE): capturing mRNA and DNA from a single sample.Analytical chemistry · 2013Article
- A Portable, Pressure Driven, Room Temperature Nucleic Acid Extraction and Storage System for Point of Care Molecular Diagnostics.Analytical methods : advancing methods and applications · 2013Article
- Modular microfluidic system fabricated in thermoplastics for the strain-specific detection of bacterial pathogens.Lab on a chip · 2012Article
- Design and development of a field-deployable single-molecule detector (SMD) for the analysis of molecular markers.The Analyst · 2012Article
- Flexible fabrication and applications of polymer nanochannels and nanoslits.Chemical Society reviews · 2011Review
- One-step purification of nucleic acid for gene expression analysis via Immiscible Filtration Assisted by Surface Tension (IFAST).Lab on a chip · 2011Article
- Purification of HIV RNA from serum using a polymer capture matrix in a microfluidic device.Analytical chemistry · 2011Article
- A simple method of fabricating mask-free microfluidic devices for biological analysis.Biomicrofluidics · 2010Article
- Polydimethylsiloxane-based conducting composites and their applications in microfluidic chip fabrication.Biomicrofluidics · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
We report a simple and effective method for the high-throughput purification of a variety of nucleic acids (NAs) from whole cell lysates or whole blood using a photactivated polycarbonate solid-phase reversible immobilization (PPC-SPRI) microfluidic chip. High-throughput operation was achieved by placing 96 purification beds, each containing an array of 3800 20 microm diameter posts, on a single 3" x 5" polycarbonate (PC) wafer fabricated by hot embossing. All beds were interconnected through a common microfluidic network that permitted parallel access through the use of a vacuum and syringe pump for delivery of immobilization buffer (IB) and effluent. The PPC-SPRI purification was accomplished by condensation of NAs onto a UV-modified PC surface in the presence of the IB comprised of polyethylene glycol, NaCl, and ethanol with a composition dependent on the length of the NAs to be isolated and the identity of the sample matrix. The performance of the device was validated by quantification of the recovered material following PCR (for DNA) or RT-PCR (for RNA). The extraction bed load capacity of NAs was 206 +/- 93 ng for gDNA and 165 +/- 81 ng for TRNA from Escherichia coli. Plate-to-plate variability was found to be 35 +/- 10%. The purification process was fast (<30 min) and easy to automate, and the low cost of wafer fabrication makes it appropriate for single-use applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.