ArticleThe EMBO journal2008
The fission yeast homologue of CENP-B, Abp1, regulates directionality of mating-type switching.
Article in The EMBO journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 21 citations in OpenAlex.
- Fission yeast Swi2 designates cell-type specific donor and stimulates Rad51-driven strand exchange for mating-type switching.Nucleic acids research · 2023Article
- The Fission Yeast Mating-Type Switching Motto: "One-for-Two" and "Two-for-One".Microbiology and molecular biology reviews : MMBR · 2023Review
- Nuclear Envelope Proteins Modulating the Heterochromatin Formation and Functions in Fission Yeast.Cells · 2020Review
- Mating-type switching by homology-directed recombinational repair: a matter of choice.Current genetics · 2019Review
- New insights into donor directionality of mating-type switching in Schizosaccharomyces pombe.PLoS genetics · 2018Article
- Molecular signature of the imprintosome complex at the mating-type locus in fission yeast.Microbial cell (Graz, Austria) · 2018Article
- The fission yeast CENP-B protein Abp1 prevents pervasive transcription of repetitive DNA elements.Biochimica et biophysica acta · 2016Article
- A Unique DNA Recombination Mechanism of the Mating/Cell-type Switching of Fission Yeasts: a Review.Microbiology spectrum · 2014Review
- Two portable recombination enhancers direct donor choice in fission yeast heterochromatin.PLoS genetics · 2013Article
- How to choose the right mate.PLoS genetics · 2013Article
- CENP-B cooperates with Set1 in bidirectional transcriptional silencing and genome organization of retrotransposons.Molecular and cellular biology · 2012Article
- Article
- A homolog of male sex-determining factor SRY cooperates with a transposon-derived CENP-B protein to control sex-specific directed recombination.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Cooperative and antagonistic contributions of two heterochromatin proteins to transcriptional regulation of the Drosophila sex determination decision.PLoS genetics · 2011Article
- Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In fission yeast, mating-type switching involves replacing genetic information contained at the expressed mat1 locus by that of either the mat2P or mat3M donor loci. Donor selection is nonrandom, as mat1P cells preferentially use mat3M for switching, whereas mat1M cells use mat2P. Switching directionality is determined by the cell-type-specific distribution of the Swi2-Swi5 complex that, in mat1P cells, localises to mat3M and, only in mat1M cells, spreads to mat2P in a heterochromatin-dependent manner. Mechanisms regulating spreading of Swi2-Swi5 across heterochromatin are not fully understood. Here, we show that the fission yeast homologue of CENP-B, Abp1, binds to the silent domain of the mating-type locus and regulates directionality of switching. Deletion of abp1 prevents utilisation of mat2P, as when heterochromatin is disrupted and spreading of Swi2-Swi5 is impaired. Our results show that, indeed, deletion of abp1 abolishes spreading of Swi2-Swi5 to mat2P. However, in abp1Delta cells, heterochromatin organisation at the mating-type locus is preserved, indicating that Abp1 is actually required for efficient spreading of Swi2-Swi5 through heterochromatin. Cbh1 and Cbh2, which are also homologous to CENP-B, have only a minor contribution to the regulation of directionality of switching, which is in contrast with the strong effects observed for Abp1.
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