ArticleJournal of clinical microbiology2008
Simultaneous genotyping of all hemagglutinin and neuraminidase subtypes of avian influenza viruses by use of padlock probes.
Article in Journal of clinical microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 37 citations in OpenAlex.
- Determination of Hemagglutinin and Neuraminidase Genetic Variation of Human Influenza A(H1N1)pdm09 Virus Strain Isolated From Deceased Patients in Shiraz, Iran: A Cross-Sectional Study.Health science reports · 2025Article
- Rolling Circle Amplification in Integrated Microsystems: An Uncut Gem toward Massively Multiplexed Pathogen Diagnostics and Genotyping.Accounts of chemical research · 2021Review
- Molecular approaches to recognize relevant and emerging infectious diseases in animals.Methods in molecular biology (Clifton, N.J.) · 2015Article
- Evolving gene targets and technology in influenza detection.Molecular diagnosis & therapy · 2013Review
- SYBR green-based real-time reverse transcription-PCR for typing and subtyping of all hemagglutinin and neuraminidase genes of avian influenza viruses and comparison to standard serological subtyping tests.Journal of clinical microbiology · 2012Article
- Colorimetric nucleic acid testing assay for RNA virus detection based on circle-to-circle amplification of padlock probes.Journal of clinical microbiology · 2011Article
- Use of consensus sequences for the design of high density resequencing microarrays: the influenza virus paradigm.BMC genomics · 2010Article
- Emerging molecular assays for detection and characterization of respiratory viruses.Clinics in laboratory medicine · 2009Review
- Molecular analysis and characterization of swine and human influenza viruses isolated in Hungary in 2006–2007.Virus genes · 2009Article
- Amplification of four genes of influenza A viruses using a degenerate primer set in a one step RT-PCR method.Journal of virological methods · 2009Article
- Use of reverse transcriptase PCR to subtype N1 to N9 neuraminidase genes of avian influenza viruses.Journal of clinical microbiology · 2009Article
- Advances in viral disease diagnostic and molecular epidemiological technologies.Expert review of molecular diagnostics · 2009Review
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A subtyping assay for both the hemagglutinin (HA) and neuraminidase (NA) surface antigens of the avian influenza virus (AIV) has been developed. The method uses padlock probe chemistry combined with a microarray output for detection. The outstanding feature of this assay is its capability to designate both the HA and the NA of an AIV sample from a single reaction mixture. A panel of 77 influenza virus strains was tested representing the entire assortment of the two antigens. One hundred percent (77/77) of the samples tested were identified as AIV, and 97% (75/77) were subtyped correctly in accordance with previous examinations performed by classical diagnostic methods. Testing of heterologous pathogens verified the specificity of the assay. This assay is a convenient and practical tool for the study of AIVs, providing important HA and NA data more rapidly than conventional methods.
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